【摘要】 分别采用单因素实验和Box-Behnken响应面法对阿里红总多糖水提醇沉工艺条件进行优化。以苯酚-硫酸法测定阿里红总多糖含量,在单因素实验基础上,选择多糖提取率为评价指标,以浸泡时间、提取时间、加水倍量作为考察因素,采用Box-Behnken响应面法优化水提工艺条件;选择多糖含量为评价指标,以醇沉浓度、醇沉时间、浓缩倍数为考察因素,采用Box-Behnken响应面法优化醇沉工艺条件;采用Sevage法对阿里红粗多糖进行脱蛋白。得到最佳水提工艺条件为:加水10倍,浸泡时间1.5 h,提取1.0 h,提取1次;最佳醇沉工艺条件为:浓缩倍数10倍,醇沉浓度80%,醇沉时间12 h;Sevage法脱蛋白,蛋白脱除率为42.4%,多糖保留率为61.4%。建立的阿里红总多糖提取纯化工艺简单可靠。更多还原

【Abstract】 We optimize the process of water extraction and alcohol precipitation of Fomes officinalis Ames total polysaccharide(FOPS) by single-factor experiments and response surface methodology.On the basis of single-factor experiments,using extraction rate of polysaccharide determined by phenol-sulfuric acid method as the evaluation index,and soaking time,extraction time,and water addition amount as the evaluation factors,we optimize the water extraction process by Box-Behnken response surface methodology.Using the content of polysaccharide as the evaluation index,alcohol precipitation time and concentration,concentration multiples as the evaluation factors,we optimize the alcohol precipitation process by Box-Behnken response surface methodology.Moreover,FOPS is deproteinized by Sevage method.The optimal water extraction process is to extract 1.0 h for once whiling soaking 1.5 h and adding 10 times of water.The optimal alcohol precipitation process is to precipitate for 12 h adding ethanol content of 80% while concentration multiples of 10 times.The deproteinization rate is 42.4% and the polysaccharide retention rate is 61.4%,which is consistent with the predicted value.This method is stable and reliable and it can be used to extract FOPS.更多还原