【摘要】 目的研究过表达miR-29a-3p对IL-22诱导的HaCaT细胞增殖的影响。方法将HaCaT细胞分为Cell组、IL-22组、IL-22+NC组和IL-22+miR-29a-3p组,荧光定量PCR检测miR-29a-3p的表达水平,CCK8检测细胞的活力,流式细胞仪检测细胞凋亡及周期。结果与0μg/L组相比,25μg/L组、50μg/L组和100μg/L组HaCaT细胞的增殖率在24 h、48 h和72 h均出现升高(F值分别为33. 27、36. 19、52. 29,均P <0. 000 1)。与0μg/L组相比,miR-29a-3p在50μg/L组和100μg/L组HaCaT中的表达水平降低(F=129,P <0. 000 1),分别降低83%和80%。与IL-22+NC组相比,IL-22+miR-29a-3p组的增殖率在24 h、48 h和72 h均降低(P值分别为0. 002 1、0. 001 6、0. 023 1),细胞总凋亡率增加(6. 67±1. 06 vs 30. 55±1. 86,P=0. 000 1),G1期细胞比例增加(P=0. 000 1),S期细胞比例降低(P=0. 000 1)。结论 IL-22可降低HaCaT中miR-29a-3p的表达量,过表达miR-29a-3p通过促进凋亡和引起细胞G1期阻滞抑制IL-22诱发的HaCaT细胞过度增生。更多还原

【Abstract】 Objective To investigate the effect of miR-29a-3p overexpression on IL-22-induced proliferation of HaCaT cells. Methods HaCaT cells were divided into four groups,Cell group,IL-22 group,IL-22 + NC group and IL-22+ miR-29a-3p group. The expression level of miR-29 a-3 p was detected by fluorescence quantitative PCR. Cell viability was detected by CCK8. Apoptosis and cell cycle were detected by flow cytometry. Results Compared with the 0 g/L group,the proliferation rate of HaCaT cells in the 25 μg/L group,50 μg/L group and 100 μg/L group was increased at 24 h,48 h and72 h( F value was 33. 27,36. 19,52. 29,respectively,all P < 0. 000 1). Compared with the 0 μg/L group,miR-29a-3p expression level in HaCaT in 50 μg/L and 100 μg/L groups was decreased( F = 129,P < 0. 000 1),with a decrease of 83%and 80%,respectively. Compared with the IL-22 + NC group,proliferation rate of IL-22 + miR-29 a-3 p group was decreased at 24 h,48 h and 72 h( P value was 0. 002 1,0. 001 6,0. 023 1,respectively),total apoptosis rate was increased( 6. 67 ±1. 06 vs 30. 55 ± 1. 86,P = 0. 000 1),cell proportion in G1 phase was increased( P = 0. 000 1),and the cell proportion in S phase was decreased( P = 0. 000 1). Conclusion Il-22 can reduce miR-29a-3p expression level in HaCaT,and miR-29a-3p overexpression can inhibit the excessive proliferation induced by IL-22 in HaCaT cells by promoting apoptosis and inducing G1 phase arrest.更多还原