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假蒟提取物生物合成纳米银及其抑菌活性研究

Biosynthesis and Characterization of Silver Nanoparticles from the Extract of Piper Sarmentosum Roxb. and Its Antibacterial Properties

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【作者】 蒋利荣黄柏华韦正柏景坤黄芳玉覃玥

【Author】 JIANG Li-rong;HUANG Bai-hua;WEI Zheng;BAI Jing-kun;HUANG Fang-yu;QIN Yue;College of Chemistry and Bio-Engineering, Hechi University;Guangxi Key Laboratory of Microbiology and Plant Resources Development;

【通讯作者】 覃玥;

【机构】 河池学院化学与生物工程学院广西高校微生物与植物资源开发重点实验室

【摘要】 假蒟提取物生物合成纳米银,并对其抑菌性能进行研究。利用紫外可见分光光度计、X射线晶体衍射、扫描电镜对PE-AgNPs进行表征,并考察其对菌株的抑菌活性。PE-AgNPs为大小均匀的球形颗粒,粒度范围在40~60 nm,抑菌活性强于假蒟提取物,可使大肠杆菌和金黄色葡萄球菌发生形态学变化,其可能的机制是纳米银渗透至细菌体内,并破坏细胞膜结构,进而使细胞内容物渗出而导致细胞破裂死亡。

【Abstract】 Silver nanoparticles were biosynthesized by using the extract of Piper sarmentosum Roxb., and the antibacterial properties were studied. The obtained PE-AgNPs were characterized by UV-visible spectrum, SEM and XRD. In addition, the antibacterial properties of the obtained PE-AgNPs were assessed in vitro by the bacteriostatic experiments and SEM. PE-AgNPs were sub-spherical with particle size ranging from 40 to 60 nm, and the antibacterial activity was stronger than the extract of Piper sarmentosum Roxb. It can cause the morphological changes of Escherichia coli and Staphylococcus aureus, and the possible mechanism is that the nano-silver penetrates infiltrate into the bacteria and destroy the cell membrane structure, then cause cell contents to exude, leading to cell rupture and death.

【关键词】 纳米银假蒟生物合成
【Key words】 silver nanoparticlesPiper sarmentosum Roxb.biosynthesis
【基金】 2019年大学生创新创业项目(SJ2019033,201910605087);广西高校中青年教师基础能力提升项目(No.KY2016LX281);河池学院校级科研课题(No.2014QN-N005);河池学院校级重点实验室项目“红水河流域药用资源综合开发利用”,河池学院高层次人才科研启动费项目(No.XJ2018GKQ014);广西高校微生物及植物资源开发利用重点实验室开放课题(No.2015HL011)
  • 【文献出处】 广州化工 ,Guangzhou Chemical Industry , 编辑部邮箱 ,2020年10期
  • 【分类号】O614.122;TB383.1
  • 【被引频次】1
  • 【下载频次】254
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