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假蒟提取物生物合成纳米银及其抑菌活性研究
Biosynthesis and Characterization of Silver Nanoparticles from the Extract of Piper Sarmentosum Roxb. and Its Antibacterial Properties
【摘要】 假蒟提取物生物合成纳米银,并对其抑菌性能进行研究。利用紫外可见分光光度计、X射线晶体衍射、扫描电镜对PE-AgNPs进行表征,并考察其对菌株的抑菌活性。PE-AgNPs为大小均匀的球形颗粒,粒度范围在40~60 nm,抑菌活性强于假蒟提取物,可使大肠杆菌和金黄色葡萄球菌发生形态学变化,其可能的机制是纳米银渗透至细菌体内,并破坏细胞膜结构,进而使细胞内容物渗出而导致细胞破裂死亡。
【Abstract】 Silver nanoparticles were biosynthesized by using the extract of Piper sarmentosum Roxb., and the antibacterial properties were studied. The obtained PE-AgNPs were characterized by UV-visible spectrum, SEM and XRD. In addition, the antibacterial properties of the obtained PE-AgNPs were assessed in vitro by the bacteriostatic experiments and SEM. PE-AgNPs were sub-spherical with particle size ranging from 40 to 60 nm, and the antibacterial activity was stronger than the extract of Piper sarmentosum Roxb. It can cause the morphological changes of Escherichia coli and Staphylococcus aureus, and the possible mechanism is that the nano-silver penetrates infiltrate into the bacteria and destroy the cell membrane structure, then cause cell contents to exude, leading to cell rupture and death.
- 【文献出处】 广州化工 ,Guangzhou Chemical Industry , 编辑部邮箱 ,2020年10期
- 【分类号】O614.122;TB383.1
- 【被引频次】1
- 【下载频次】254