【摘要】 目的探讨甲状腺肿瘤患者PCDH8基因启动子的甲基化状态及其临床意义。方法选取48例甲状腺乳头状癌(PTC)、43例甲状腺滤泡状腺瘤、44例结节性甲状腺肿和45例正常甲状腺组织标本。采用焦磷酸测序法检测各组标本PCDH8基因的启动子甲基化状态,采用免疫组化检测各组标本的PCDH8蛋白表达情况,应用McNemar检验焦磷酸测序和免疫组化检测结果的一致性,并分析PCDH8基因启动子甲基化与PTC临床病理特征的关系。结果 PTC的PCDH8基因启动子甲基化率高于正常甲状腺组织,PCDH8基因蛋白表达阳性率低于正常甲状腺组织(均P<0.05),而甲状腺滤泡状腺瘤、结节性甲状腺肿及正常甲状腺组织的上述指标差异均无统计学意义(均P>0.05)。PCDH8基因发生甲基化与基因蛋白表达缺失在PTC的重合率为89.18%,两者的一致性检验结果差异无统计学意义(P>0.05)。PTC患者的PCDH8基因启动子甲基化与淋巴结转移有相关性(P<0.05),而与其年龄、性别、促甲状腺激素水平、抗甲状腺球蛋白抗体水平及肿瘤部位无明显关系(均P>0.05)。结论 PTC中PCDH8基因启动子甲基化率高,PCDH8蛋白表达水平低,PCDH8基因甲基化与蛋白表达调控具有一定的关联性,且PTC患者的PCDH8基因启动子甲基化与淋巴结转移有相关性。更多还原

【Abstract】 Objective To investigate the methylation status of PCDH8 gene promoter and its clinical significance in patients with thyroid tumors.Methods The specimens of 48 cases of papillary thyroid carcinoma(PTC), 43 cases of thyroid follicular adenoma, 44 cases of nodular goiter and 45 cases of normal thyroid tissues were selected. The promoter methylation status of PCDH8 gene in specimens of each group was detected using the sequenced method by detecting pyrophosphate release, the expression of PCDH8 protein in specimens of each group was detected by immunohistochemistry, McNemar test was applied to assessing the coincidence of detection results between the sequenced method by detecting pyrophosphate release and immunohistochemistry, and the relationship between promoter methylation of PCDH8 gene and the clinicopathological features of PTC was analyzed.Results PTC exhibited higher methylation rate of PCDH8 gene promoter and lower positive protein expression rate of PCDH8 gene as compared with normal thyroid tissue(all P<0.05), but there was no statistically significant difference in the aforementioned indices among thyroid follicular adenoma, nodular goiter and normal thyroid tissue(all P>0.05). The coincidence rate of methylation of PCDH8 gene and protein expression loss of PCDH8 gene was 89.18% in PTC, no statistically significant difference was found in the coincidence between the results of the two detections(P>0.05). PCDH8 gene promoter methylation was associated with lymph node metastasis in PTC patients(P<0.05), but not significantly with age, gender, thyroid-stimulating hormone level, anti-thyroglobulin antibody level or tumor site(all P>0.05).Conclusion The methylation rate of PCDH8 gene promoter is high and the expression level of PCDH8 protein is low in PTC. A certain association exists between the methylation of PCDH8 gene and the regulation of PCDH8 protein expression, and the methylation of PCDH8 gene promoter correlates with lymph node metastasis in PTC patients.更多还原