【摘要】 目的:探索脂多糖(LPS)干预原代小胶质细胞时间与表达诱导型一氧化氮合酶(iNOS)时效关系的研究,为小胶质细胞建立炎症模型提供依据。方法:把提纯后的原代小胶质细胞随机分为实验组和对照组。实验组分别用LPS干预6 h、12 h、24 h和48 h,对照组为LPS未干预组,即LPS干预0 h。用免疫荧光和蛋白质免疫印迹法(Western blot)分别检测iNOS在小胶质细胞中的表达。结果:实验组LPS干预小胶质细胞6 h、12 h、24 h和48 h后iNOS的表达量增高,其中12 h,24 h,48 h与对照组0 h比较,差异有统计学意义(P<0.01),且在24 h前逐渐升高,24 h达峰值,24 h后逐渐下降。结论:iNOS表达量与LPS干预原代小胶质细胞的时间有关,LPS干预24 h时iNOS表达最高,LPS干预24 h为理想的小胶质细胞炎症模型。更多还原

【Abstract】 Objective: To explore the time-efficien relationship between the lipopolysaccharide(LPS) intervention in primary microglia and the expression of inducible nitric oxide synthase(iNOS), and to provide evidence for the establishment of inflammation model of microglia. Methods: The purified primary microglia was randomly divided into experimental group and control group. The experimental group was treated with LPS for 6 h, 12 h,24 h and 48 h respectively, and the control group was LPS non-intervention group(no LPS intervention). The expression of iNOS in microglia was detected by immunofluorescence and Western blot. Results: The expression of iNOS in microglia was increased after LPS intervention for 6, 12, 24 and 48 h in the experimental group,among which, the difference was statistically significant when compared with the control group for 0 h at 12 h,24 h and 48 h(P<0.01). The expression of iNOS was gradually increased before 24 h, reached the peak at 24 h,and then gradually decreased after 24 h. Conclusion: The expression of iNOS is related to the LPS intervention time in primary microglia. The expression of iNOS will reach the highest level after LPS intervention for 24 h,and microglia treated with LPS for 24 h is the ideal microglial inflammation model.更多还原