【摘要】 目的:探究毛蕊异黄酮对大鼠脑缺血再灌注损伤的保护作用机制。方法:将80只SD大鼠随机分为假手术组(sham)、缺血再灌注组(I/R)、低剂量组、中剂量组、高剂量组。在造模前14 d,低剂量组、中剂量组、高剂量组分别腹腔注射5 mg/(kg·d)、10 mg/(kg·d)、20 mg/(kg·d)毛蕊异黄酮,sham组和I/R组等量注射二甲基亚砜,随后建立脑缺血再灌注模型,24 h后评估大鼠神经功能评分,计算脑梗死体积及脑含水量,测定脑组织中超氧化物歧化酶(SOD)和丙二醛(MDA)的含量及NF-κB表达量。结果:低剂量组、中剂量组、高剂量组的神经功能评分、脑梗死体积、脑含水量随着毛蕊异黄酮浓度升高而降低,以高剂量组下降最为明显(P<0.05或P<0.01),均低于I/R组(P<0.05或P<0.01)。低剂量组、中剂量组、高剂量组的脑组织中SOD活性显著高于I/R组(P<0.01),MDA水平显著低于I/R组(P<0.01);低剂量组、中剂量组、高剂量组的SOD活性随着毛蕊异黄酮浓度的增加而升高(P<0.05或P<0.01),低剂量组、中剂量组、高剂量组的MDA水平随着毛蕊异黄酮浓度升高而降低(P<0.05或P<0.01)。与I/R组相比,低剂量组、中剂量组、高剂量组的NF-κB的表达显著下调(P<0.01);低剂量组、中剂量组、高剂量组的NF-κB的表达随着毛蕊异黄酮浓度升高而下调,以高剂量组下调最为明显(P<0.05或P<0.01)。结论:毛蕊异黄酮对大鼠脑缺血再灌注损伤具有保护作用。更多还原

【Abstract】 Objective: To explore the protective mechanism of calycosin on cerebral ischemia reperfusion injury in rats. Methods: Eighty SD rats were randomly divided into the groups sham operation, ischemia/reperfusion(I/R), low-dose, medium-dose and high-dose. Fourteen days before model establishment, the rats of low-dose, middle-dose, and high-dose groups were intraperitoneally injected with 5 mg/(kg·d), 10 mg/(kg·d),and 20 mg/(kg·d) calycosin, respectively; the sham and I/R group rats were injected with an equal amount of dimethyl sulfoxide. Next the cerebral ischemia and reperfusion models were constructed. Twenty-four hours later, the neurological function score of rats was evaluated, the cerebral infarction volume and brain water content were calculated, and the superoxide dismutase(SOD) and malondialdehyde(MDA) content and NF-κB expression in the brain tissue were determined. Results: The neurological function score, cerebral infarction volume, and brain water content of the low-dose, medium-dose, and high-dose groups decreased with the increase of calycosin concentration, with the high-dose group showing the most significant decrease(P<0.05 or P<0.01), and all showed lower values than the I/R group(P<0.05 or P<0.01). The SOD activity in the brain tissue of the low-dose, medium-dose, and high-dose groups was significantly higher than that of the I/R group(P<0.01), and the MDA level was significantly lower than that of the I/R group(P<0.01); the SOD activity of the low-dose, medium-dose, and high-dose groups increased with the increase of calycosin concentration(P<0.05 or P<0.01); the MDA level of the low-dose, medium-dose, and high-dose groups decreased with the increase of calycosin concentration(P<0.05 or P<0.01). Compared with the I/R group, the expression of NF-κ B in the low-dose, medium-dose, and high-dose groups was significantly down-regulated(P<0.01); the expression of NF-κB in the low-dose, medium-dose, and high-dose groups was down-regulated with the increase of calycosin concentration, with the high-dose group showing the most significant down-regulation(P<0.05 or P<0.01).Conclusion: Calycosin has protective effects in cerebral ischemia reperfusion rats.更多还原