【摘要】 目的探讨利拉鲁肽（liraglutide）对MC3T3-E1前成骨细胞增生分化的影响及其机制。方法体外培养MC3T3-E1前成骨细胞,给予不同浓度的利拉鲁肽(0、10^-6、10^-7、10^-8、10^-9mol/L),24 h后用CCK8法检测细胞的增生活性,48 h后用碱性磷酸酶试剂盒测定碱性磷酸酶（alkaline phosphatase,ALP）活力;给予最适浓度10^-7mol/L利拉鲁肽处理MC3T3-E1细胞0、1、3、5、10 h,实时定量PCR技术检测BIP、XBP1基因的表达水平。结果与对照组相比,10^-6、10^-7、10^-8、10^-9mol/L利拉鲁肽组细胞增生活性A值分别升高了0.042、0.073、0.038、0.032 （P<0.05）,10^-7mol/L利拉鲁肽组A值升高最显著;与对照组相比,10^-6、10^-7、10^-8、10^-9mol/L利拉鲁肽组ALP活性分别升高了0.400、0.717、0.321、0.303 （P<0.05）,10^-7mol/L利拉鲁肽组ALP活性升高最显著。与0 h比较,10^-7mol/L利拉鲁肽处理后,BIP基因的表达随时间的增加而增高,1和3 h无显著变化（P>0.05）,5 h （1.983±0.230,P<0.01）和10 h （2.469±0.256,P<0.01）表达明显增加;XBP1基因表达在1 h无显著变化（P>0.05）,3 h显著升高（3.449±0.246,P<0.01）,5 h有下降趋势（1.597±0.259,P<0.01）,10 h又升高（2.412±0.274,P<0.01）。结论利拉鲁肽可能通过上调BIP、XBP1基因的表达,促进MC3T3-E1细胞的增生与分化。更多还原

【Abstract】 Objective To explore the roles and mechanism of liraglutide on the proliferation and differentiation of the MC3T3-E1 cells.Methods MC3T3-E1 cells were cultured in vitro with different concentrations of liraglutide (0,10^-6,10^-7,10^-8,10^-9mol/L),after 24 h cell proliferation was detected with cell counting kit-8 （CCK8）,alkaline phosphatase （ALP） activity was determined using the ALP kit after 48 h.Cultures were treated for 0,1,3,5,and 10hours with the optimal concentration of liraglutide (10^-7mol/L),and the expression of BIP and XBP1 mRNA were measured by the qRT-PCR.Results Compared with the control group,the A values of 10^-6,10^-7,10^-8,10^-9mol/L liraglutide groups increased respectively by 0.042,0.073,0.038,0.032 （P<0.05）.The A values of 10^-7mol/L liraglutide group increased most significantly.Compared with the control group,the ALP activity of 10^-6,10^-7,10^-8,10^-9mol/L liraglutide groups increased respectively by 0.400,0.717,0.321,0.303 （P<0.05）.Liraglutide (10^-7mol/L) had the strongest effects.After liraglutide (10^-7mol/L) treatment,compared to unstimulated cells （time 0）,the expression of BIP mRNA increased with time.BIP mRNA levels were not altered at 1 h and 3 h （P>0.05）,increased significantly at 5 h（1.983±0.230,P<0.01） and 10 h （2.469±0.256,P<0.01）.The expression of XBP1 mRNA was not altered at 1 h（P>0.05）,and increased significantly at 3 h （3.449±0.246,P<0.01）,decreasing at 5 h （1.597±0.259,P<0.01）,increased at 10 h again （2.412±0.274,P<0.01）.Conclusion Liraglutide could promote the proliferation and differentiation of MC3T3-E1 cells through up-regulating the mRNA expression of BIP and XBP1.更多还原