【摘要】 根据目前的研究现状,叶绿素荧光是植物生理学研究和植物遥感研究的重要手段之一。前人对激光诱导荧光（LIF）光谱研究多采用垂直方向,较少考虑多角度及荧光偏振变化的影响。对此分别以吊兰、绿萝、金边虎皮兰作为研究对象,采用多角度及偏振观测方式对激光诱导叶绿素荧光光谱进行多因素分析并建立模型。同时以极坐标的方式绘制激光诱导叶绿素荧光强度与相应偏振度的2π空间分布。结果表明,三种植物荧光光谱形状各不相同,且观测天顶角与相对方位角的改变影响荧光强度和荧光参数大小。在不同相对方位角上建立观测天顶角与荧光参数F₆₈₅/F₇₄₀的数学回归模型。根据比较R²与RMSE,相对方位角为225°是同时研究这三种植被F₆₈₅/F₇₄₀值与观测天顶角关系的最佳方位角,其回归系数显著,可以准确反应三种植被F₆₈₅/F₇₄₀与观测天顶角有显著的相关关系。此外,我们还对3种植物的激光诱导叶绿素荧光进行偏振观测,并计算其在2π空间中对应的荧光偏振度。研究表明,激光诱导叶绿素荧光光谱具有显著的偏振性,观测天顶角、相对方位角、波段等信息都对激光诱导叶绿素荧光偏振光谱有着不同程度的影响。针对不同植被,建立不同波段荧光偏振度关系的数学回归模型,其回归系数显著,可以准确反应不同波段荧光偏振度有显著的相关关系。更多还原

【Abstract】 According to the current research status, chlorophyll fluorescence is one of the important means of plant physiology research and plant remote sensing research. Previous studies on laser-induced fluorescence（LIF） have mostly used vertical directions, and less consideration has been given to the effects of multi-angle and fluorescence polarization. In this paper, Chlorophytum, Green radish, and Phnom Penh Tiger Piran were used as research objects, and multi-angle and polarization observation methods were used to analyze the laser-induced chlorophyll fluorescence spectra, and the models were established. At the same time, the spatial distribution of laser-induced chlorophyll fluorescence intensity and degree of polarization was plotted in polar coordinates. The results show that the shapes of fluorescence spectra of the three plants were different, and the changes in the Viewing Zenith Angles and Relative Azimuth Angles affect the fluorescence intensity and fluorescence parameters. The mathematical regression models of Viewing Zenith Angles and fluorescence parameter F₆₈₅/F₇₄₀ were established at Relative Azimuth Angles. According to the comparison of R² and RMSE of different models, the Relative Azimuth Angle of 225° is the best angle to study the relationship between F₆₈₅/F₇₄₀ and Viewing Zenith Angles for these three plants at the same time. The regression coefficient is significant, which can accurately reflect that F₆₈₅/F₇₄₀ has a significant correlation with Viewing Zenith Angles. In addition, we also performed polarization observations on the laser-induced chlorophyll fluorescence of the three plants and calculated the corresponding fluorescence polarization in the 2π space. The studies have shown that the laser-induced chlorophyll fluorescence spectrum has significant polarization, and the Viewing Zenith Angles, Relative Azimuth Angles, and band information have different effects on laser-induced chlorophyll fluorescence polarization spectra. The mathematical regression models were established for the relationship between the fluorescence polarization at 685 and 740 nm for different plants. The regression coefficient is significant, which could accurately reflect the significant correlation between the fluorescence polarization at 685 and 740 nm.更多还原