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废水中Hg2+快速检测试纸条研制

Development of Rapid Test Strip for Hg2+ in Wastewater

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【作者】 铁文利王莉李春光彭赵旭

【Author】 TIE Wenli;WANG Li;LI Chunguang;PENG Zhaoxu;College of Ecology and Environment,Zhengzhou University;Zhengzhou University of Aeronautics,Key Laboratory of Environmental Functional Meterial;

【通讯作者】 李春光;

【机构】 郑州大学生态与环境学院郑州航空工业管理学院环境功能材料重点实验室

【摘要】 为了探索快速检测Hg2+的方法,采用水热法制备中空球状的CeO2纳米材料。利用CeO2具有生物酶活性能使四甲基联苯胺(TMB)显色的特点,以DNA适配体和铈基材料构建Hg2+的检测技术,并对试纸条检测条件进行优化。结果表明当CeO2浓度为10 mg/mL,DNA适配体浓度为25μmol/L时检测效果最好。通过检测溶液中的Cd2+、Ni2+、Cr3+,得到具有专一性的CeO2纳米材料制备的试纸条,开发出新的检测Hg2+的有效方法。

【Abstract】 In order to explore the method of rapid detection of Hg2+, hollow spherical nano-CeO2materials were prepared by hydrothermal method.Utilizing the characteristics of CeO2 with biological enzyme activity to make TMB(tetramethylbenzidine) color,the Hg2+ detection technology was constructed with DNA aptamer and cerium-based materials,and the test strip detection conditions were optimized.The results show that the detection effect is best when the concentration of CeO2 is 10 mg/mL and the concentration of DNA aptamer is 25 μmol/L.By detecting Cd2+,Ni2+ and Cr3+ in the solution,a test strip made of specific nano-CeO2materials was obtained,and a new effective method for detecting Hg2+ was developed.

【关键词】 Hg2+DNA适配体试纸条纳米CeO2
【Key words】 Hg2+DNA aptamerTest stripNano-CeO2material
【基金】 国家水体污染控制与治理科技重大专项(No.2015ZX07204-002-005)
  • 【文献出处】 分析科学学报 ,Journal of Analytical Science , 编辑部邮箱 ,2020年03期
  • 【分类号】X853;O657.3
  • 【下载频次】120
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