【摘要】 以吲哚磺酸盐与不同结构的芳香醛为原料,合成了检测HSO^-₃的3种新型荧光探针ISBD、ISPD和ISND,并对筛选出的性能更优的双功能（HSO^-₃/pH）荧光探针ISND进行深入研究。检测可在1 min内快速完成,检出限为8.11×10^-8 mol/L。干扰实验结果表明,包括F^-、Cl^-、Br^-、I^-、NO^-₃、HCO^-₃、S^2-、SCN^-、ClO^-、NO^-₂、C₂O■、OAc^-、N^-₃、Cys、Hcy、GSH和CN^-在内的17种干扰物质对HSO^-₃的检测基本没有影响。同时,探针ISND具有细胞毒性低、细胞膜通透性好等优点,可实现对细胞内HSO^-₃的检测及成像。同ISBD相比,当芳香醛中引入羟基后,探针ISPD和ISND能够通过两个不同的发射波长完成对HSO^-₃（ISPD为385 nm,ISND为458 nm）以及pH值（ISPD为560 nm,ISND为565 nm）的差异性双重检测,在传感分析、生物信号传导及检测方面具有良好的应用前景。更多还原

【Abstract】 Three new fluorescent probes（ISBD, ISPD and ISND） were designed and synthesized. The UV-visible absorption spectrum, fluorescence spectrum, and response to pH values of the probe were studied, and the HSO^-₃/pH dual detection fluorescent probe ISND with stronger fluorescence intensity（higher fluorescence quantum yield）, longer emission wavelength and less interference was selected for the further study. The probe ISND could be used for the sensitive and specific detection of HSO^-₃, with a detection limit of 8.11 × 10^-8mol/L(λ_em=458 nm, response time <1 min). In addition, with different emission wavelength(λ_em=565 nm), the probe could accurately detect pH value. Furthermore, the probe ISND had many advantages such as low toxicity and good membrane permeability. It could achieve fluorescence imaging of HSO^-₃ in living cell, and exhibited good application prospects in sensing, biological signal transduction and detection.更多还原