【摘要】 搭建了一种联用超分辨荧光和三维形貌显微成像系统(Correlative super-resolution fluorescence and three-dimensional topography imaging microscopy),以聚合纤维状肌动蛋白(F-actin)为例,评价了此联用系统的性能。实验结果表明,此联用仪器具有纳米级分辨率的三维形貌及具体成分的定位分布成像功能,可实现在形貌图中定位具体成分的功能,表明此联用仪器可应用于研究分析细胞骨架及细胞亚结构(如细胞膜蛋白质聚集体的组成和分布特性等)方面,此联用系统在生命科学和材料科学等领域具有广泛的应用前景。更多还原

【Abstract】 A correlative super-resolution fluorescence and three-dimensional(3 D) topography imaging microscopy was built, and the performance of the correlative system was evaluated with F-actin as an example. The experimental results suggested that the correlative instrument had the abilities to provide 3 D overall topography and distribution of specific components at nanometer resolution, as well as to correlate the specific components’ distribution with the overall topography. Thus, the correlative instrument is a powerful tool to investigate events regarding cytoskeleton and cellular substructure such as the composition and distribution characteristics of membrane protein clusters, and can also be widely applied to other fields such as life science and material science.更多还原