【摘要】 目的了解厦门市新发现人类免疫缺陷病毒-1（HIV-1）感染者的血浆HIV病毒载量及外周血T淋巴细胞基线情况,探讨病毒载量与外周血T淋巴细胞数量的相关性。方法采用罗氏COBAS TaqMan 48病毒载量仪和贝克曼FC500流式细胞仪检测269例新确证HIV-1感染者的血浆HIV病毒载量和外周血T淋巴细胞,应用SPSS 20软件对检测数据进行分析。结果 93.69%（252/269）样本的病毒载量≥10³拷贝/mL,病毒载量检测结果为23～1.76×10⁶ 拷贝/mL,对数平均值为（4.43±0.79）log/mL;不同性别、年龄、婚姻状况、文化程度、传播途径的新发现HIV-1感染者的病毒载量水平构成情况比较,差别无统计学意义（P>0.05）;血浆病毒载量对数值与CD3⁺T淋巴细胞总数、CD8⁺细胞计数无显著相关性,但与CD4⁺细胞数量和CD4⁺/CD8⁺比值总体呈负相关[r分别为-0.276（P<0.01）和-0.355（P<0.01）]。结论厦门市大部分新发现的HIV-1感染者体内HIV病毒复制活跃,应考虑联合应用病毒载量和外周血T淋巴细胞基线检测结果指导抗病毒治疗和观测抗病毒治疗效果。更多还原

【Abstract】 Objective To investigate the base data of plasma HIV viral load and peripheral blood T-lymphocytes in newly detected HIV-1 infected individuals in Xiamen, and discuss the correlation between viral load（VL） and the number of peripheral blood T-lymphocytes. Methods The plasma HIV viral load and peripheral blood T-lymphocytes of 269 newly confirmed HIV-1 infected patients were detected by Roche COBAS TaqMan 48 and Beckman FC500 flow cytometer. The test data were analyzed by using SPSS 20. Results Patients with HIV viral load ≥10³ copies/mL accounted for 93.69%（252/269）. The minimum VL was 23 copies/mL, and the maximum VL was 1.76×10⁶ copies/mL, with a logarithmic average of（4.43±0.79） log/mL. Different gender, age, marital status, education level, and transmission route had no statistical significance on the viral load level of newly discovered HIV-1 infected individuals（P>0.05）. The plasma viral load logarithm was not significantly correlated with CD3⁺T lymphocytes and CD8⁺ cell count, but negatively correlated with the number of CD4⁺ cells and the ratio of CD4⁺/CD8⁺, and the corresponding coefficients were-0.276（P<0.01） and-0.355（P<0.01）, respectively. Conclusion In Xiamen, the replication of HIV-1 virus is active in most newly detected HIV-1 infected individuals, so the combination of viral load and peripheral blood T-lymphocyte baseline test results should be considered to guide antiretroviral therapy and observe the effect of antiretroviral therapy.更多还原