【摘要】 【目的】研究老鹳草乙醇提取物对12株临床致病菌的抑菌活性,为开发新型植物来源的抗生素提供重要的参考。【方法】以老鹳草全草乙醇提取物为研究对象,运用气相色谱-质谱联用仪、高效液相色谱仪研究其化学成分;运用滤纸片法测试12株临床致病菌的抑菌活性。【结果】从老鹳草乙醇提取物中鉴定出:18个挥发性组分包括脂肪烃、脂肪酸和生物碱,3个黄酮化合物杨梅苷、槲皮素-3-O-β-D-葡萄糖、花旗松素。老鹳草乙醇提取物对粪链球菌(MIC 1.0 mg·mL^-1)、耐甲氧西林金黄色葡萄球菌(MIC 1.0 mg·mL^-1)、大肠杆菌(MIC 1.0 mg·mL^-1)及鲍曼不动杆菌(MIC 2.0 mg·mL^-1)有高敏抑制活性。【结论】恩施地区老鹳草乙醇提取物主要分挥发性组分为脂肪烃、脂肪酸、生物碱;含有杨梅苷、槲皮素-3-O-β-D-葡萄糖、花旗松素3个黄酮类化合物;对常见临床致病菌具有优良抗菌活性和较广的抗菌谱覆盖面。更多还原

【Abstract】 【Objective】 Antibacterial activity and mechanism of the ethanol-extract of Geranium wilfordii Maxim on clinical pathogens were studied for development of a natural antibiotic. 【Method】 The chemical compositions of the extract were analyzed by GC-MS and HPC. The filter paper method was used to test the antimicrobial activity of the extract against 12 clinical samples of the resistant bacteria. 【Result】 GC-MS detected 18 volatile substances, including aliphatic hydrocarbons,fatty acids, and alkaloids（1.15%）. The 3 flavonoid compounds identified were myricitrin, quercetin-3-O-β-D-glucose, and taxifolin. High antibacterial activities against Enterococcus faecalis ATCC 29212(MIC 1.0 mg·mL^-1), methicillin-resistant Staphylococcus aureus(MIC 1.0 mg·mL^-1), Escherichia coli ATCC 25922(MIC 1.0 mg·mL^-1), and Acinetobacter baumannii ATCC 29212(MIC 2.0 mg·mL^-1) of the extract were observed. 【Conclusion】 In the G. wilfordii ethanol-extract, aliphatic hydrocarbons, fatty acids, and alkaloids were the major volatiles, and myricitrin, quercetin-3-O-β-D-glucose, and taxifolin the3 substantial flavonoid compounds found. The extract demonstrated a significant antibacterial activity with wide spectrum against the commonly known clinical pathogens.更多还原