【摘要】 该文以浙江省某河道为研究对象,分别选取生态浮床所在位置的上中下游,通过16S rDNA高通量测序技术,研究河流上下游水质和微生物变化,分析群落变化原因。人工生物浮岛能够有效去除水体中的总氮和总磷,去除率分别达到55%和45%。细菌16S rDNA高通量测序显示Group S2组具有较高细菌群落丰富度和多样性。生态浮岛下游中的微生物丰度小于上游,微生物多样性高于上游。在上下游群落结构组成上,主要在纲和科水平发生显著变化,优势菌科从黄杆菌科、孢鱼菌科转变为丛毛单胞菌科、腐螺旋菌科。人工生物浮岛能够有效去除水体中的总氮和总磷,改变细菌群落的丰度和多样性,特别是在纲和科水平,使细菌优势菌群发生改变,造成细菌菌落演变偏移,菌落构成呈现多样性变化。更多还原

【Abstract】 Taking one river channel on Zhejiang Province as the research object, the upper, middle and lower reaches of artificial floating islands were selected respectively. The 16 S rDNA high-throughput sequencing technology was used to study the water quality and microbial changes in the upper and lower reaches of the river, and to analyze the community changes.Artificial biological floating islands can effectively remove total nitrogen and total phosphorus in water. The removal rate of total nitrogen and total phosphorus in water reaches 55% and 45%. High-throughput sequencing of bacterial 16 S rDNA indicated that Group S2 group had higher richness and diversity of bacterial communities. The microbial abundance in the downstream of artificial floating islands is smaller than that in the upstream, but the microbial diversity is higher than the upstream.In the composition of the upstream and downstream community structure, there was a significant change in the level of the class and the family. The dominant species was transformed from the genus Flavobacteriaceae and Sporichthyaceae to the genus Comamonadaceae and Saprospiraceae. The artificial floating islands can effectively remove the total nitrogen and total phosphorus from water, change the abundance and diversity of the bacterial communities, especially at the level of class and family, so that the dominant bacteria flora changes, resulting the evolution and migration of bacterial colonies. The colony composition exhibits diversity changes. The study of the dynamic changes of bacterial colony composition in this study provides some reference for the treatment of eutrophication by artificial floating islands.更多还原