【摘要】 为了研究Lmo0331蛋白在单增李斯特菌环境适应性中发挥的作用,利用同源重组的技术构建LM90SB2△lmo0331基因缺失株,比较在不同温度、pH、渗透压下的生长特性和生物被膜形成能力与野毒株的差异。结果显示,在4℃环境下及含5g/L NaCl的BHI培养基中,LM90SB△lmo0331缺失株的生长速度显著高于LM90SB2野毒株(P<0.05);在pH9的碱性环境及含45mL/L乙醇的BHI培养基中,LM90SB2△lmo0331缺失株繁殖数量低于野毒株(P<0.05);其他条件下,LM90SB△lmo0331缺失株与野毒株的生长无显著差异;LM90SB2和LM90SB2△lmo0331在不同时间点生物被膜形成密度无显著差异。由此表明,Lmo0331蛋白可能在低温和碱性条件下发挥不同作用,这一结果为研究LM在胁迫环境生存机制奠定了基础。更多还原

【Abstract】 To study the role of Lmo0331 protein on environmental tolerance of Listeria monocytogenes(LM),based on homologous recombination technology,LM90 SB2△lmo0331 deletion mutant was constructed.The growth curves of LM90 SB2 and LM90 SB2△lmo0331 under different temperatures,pH,osmotic pressure and the ability of biofilm formation were detected.The growth of LM90 SB2△lmo0331 was better than that of LM90 SB2 at 4℃ and 5 g/L NaCl(P<0.05)BHI medium.The resistant ability of LM90 SB2△lmo0331 was significantly lower than that of LM90 SB2 in the alkali(pH9)and 45 mL/L ethanol(P<0.05).The growth of LM90 SB2△lmo0331 and LM90 SB2 was not significantly different under other stress conditions.There was no significant different in biofilm formation ability between LM90 SB2 and LM90 SB2△lmo0331 at different time points.The results suggested that Lmo0331 protein of Listeria monocytogenes plays different roles under low temperature and alkalinity stress conditions,which laid the foundation for study LM in environmental tolerance.更多还原