【摘要】 目的分析吗啡线粒体毒性导致线粒体超微结构改变对家猫脑皮层异常放电的影响,探讨吗啡依赖所致脑功能损害的可能机制。方法将12只家猫按照随机数字表法分成对照组(3只)和吗啡暴露组(9只),用剂量递增法造模成功后,行大脑皮层脑电检测,电镜下观察各脑区皮质神经元线粒体的超微结构改变。结果吗啡暴露组脑电图(EEG)监测显示皮层脑电广泛异常,生理波减少,异常放电频繁出现;电镜示神经元线粒体超微结构在数量、形态、内部膜结构、基质内包涵体各方面均发生改变,表现多样。而对照组EEG及电镜表现均正常。结论吗啡可损害大脑皮层神经元,导致异常放电,这种损害与神经元线粒体超微结构改变关系密切,吗啡线粒体毒性可作为脑细胞能量代谢功能障碍并成为最终导致脑电生理功能活动紊乱的始发机制。更多还原

【Abstract】 Objective To analyze the effect of mitochondrial ultrastructural changes caused by morphine toxicity on abnormal discharge of cat cerebral cortex,and to explore the possible mechanism of brain function damage caused by morphine dependence.Methods Twelve domestic cats were divided into control group(3 cats) and morphine exposed group(9 cats) according to the method of random number table.After the model was successfully established by the method of dose increasing,the changes of mitochondrial ultrastructure of cortical neurons were observed under the electron microscope.Results Electroencephalogram(EEG) monitoring in morphine exposed group showed that the cortical EEG was widely abnormal,physiological waves were reduced,and abnormal discharges were frequent.And the electron microscopy showed that the number,morphology,internal membrane structure and the inclusion body in the matrix of neurons changed in various aspects.The EEG and electron microscopy of the control group were normal.Conclusion Morphine can damage neurons in the cerebral cortex and lead to abnormal discharge,which is closely related to the ultrastructural changes of neuron mitochondria.The toxicity of morphine mitochondria can be the initial mechanism of energy metabolism dysfunction of brain cells and eventually lead to the disorder of brain electrophysiological function.更多还原