【摘要】 传统的聚合酶链式反应仪器温度循环中高温向低温过渡的阶段,制冷性能差,导致实验周期较长。本文采用具有加热与制冷功能的帕尔贴元件,以自适应强的模糊PID作为温度控制的核心算法,并通过状态机方式切换制热与制冷功能,设计了一套实现快速变温的PCR温控模块。更多还原

【Abstract】 The transition from high temperature to low temperature in the temperature cycle of the traditional polymerase chain reaction(PCR)instrument takes a longer test period due to the lacking high effective refrigeration function.In the paper,a PCR temperature control module for rapid temperature change is designed by using the Peltier with heating and cooling function,the adaptive fuzzy PID algorithm as the core algorithm for temperature control,and the status machine as the heating and cooling switch.更多还原