【摘要】 肿瘤是一种由多种致癌基因功能的获得,抑癌基因功能的丢失以及环境因素共同决定的复杂疾病。肿瘤细胞的表型异质性、细胞生物学背景以及肿瘤微环境是决定肿瘤发展过程的关键因素。体外三维细胞培养(three-dimensional cell culture,3DCC)可以更好地模拟真实的肿瘤细胞微环境,对探索肿瘤的发生、侵袭以及转移的分子机制起到了决定性作用。本文将结合近年来相关文献,对3DCC的建立方法及其在恶性肿瘤中的应用进展作一综述。更多还原

【Abstract】 Tumor is a complex disease which is involved in a variety of oncogene activation,tumor suppressor gene deactivation and environmental factors. Phenotypic heterogeneity,biological context and microenvironment of tumor cells are the key factors to determine the development of tumor. Three-dimensional cell culture( 3 DCC) mimics the microenvironment of native tumor tissue and plays an important role in exploring the molecular mechanism of tumor initiation,invasion and metastasis. This review summarized the establishment methods of 3 DCC and its application advances in malignant tumor research.更多还原