【摘要】 目的观察穴位埋线对氯化锂—匹罗卡品致痫大鼠海马神经元损伤的保护作用。方法 80只SD雄鼠随机分为空白组、模型组、西药组及埋线组,每组20只。制造癫痫模型,分别进行相应的干预,28 d后对各组大鼠进行Racine分级评定并记录各组癫痫平均发作时间;处死取材,行HE染色及免疫组化。结果癫痫发作程度及平均发作时间:模型组大鼠可出现典型地癫痫发作征象(P<0.01);与模型组比较,西药组及埋线组发作程度明显减轻,平均癫痫发作时间明显减少(P<0.05)。海马CA1区HE染色结果:模型组大鼠存在大量调亡细胞;埋线组及西药组可见少量凋亡细胞。海马Capase-3阳性细胞数及蛋白的表达:模型组大鼠海马阳性细胞数及蛋白表达明显增多 (P<0.05);与模型组比较,埋线组与西药组均明显减少(P<0.05)。以上结果中,西药组与埋线组结果无统计学差异(P>0.05)。结论癫痫可造成大鼠海马神经元损伤,穴位埋线可以减缓海马神经细胞丢失程度,减缓细胞凋亡进程,减弱癫痫发作程度,起到脑保护作用。更多还原

【Abstract】 Objective To observe the protective effect of acupoint embedding therapy on hippocampal neuronal injury in rats with epilepsy induced by lithium chloride-pilocarpine.Methods A total of 80 healthy SD male rats were randomly divided into blank group,model group,Western medicine group and embedding group,with 20 rats in each group.After the successful modeling,the corresponding interventions were performed for 28 days.The degree of seizure and the average seizure time were recorded.Then,the hippocampus was sacrificed in each group to complete the test of HE staining and immunohistochemistry.Results The degree of seizure and the average seizure time:typical seizure signs can be observed in the model group (P<0.01);compared with the model group,the seizures degree as well as the average seizure time in the Western medicine group and the embedding group were significantly reduced (P<0.05).HE staining results in hippocampal CA1 area:the rats in the model group showed a large number of apoptotic cells;the damage degree of hippocampus cells in the embedding group and the Western medicine group was significantly lighter than that in the model group,a small number of apoptotic cells were visible.The number of Capase-3 positive cells and protein expression in hippocampus CA1 area:compared with the blank group,the number of Caspase-3 positive cells and Caspase-3 protein in the hippocampal CA1 area of the model group were significantly increased (P<0.05);after intervention,the number of positive cells and Caspase-3 protein in the embedding group and the Western medicine group were significantly decreased (P<0.05).Among the above results,there was no difference between the Western medicine group and the embedding group (P>0.05).Conclusion Epilepsy can cause neuronal damage in rat hippocampus.Acupoint embedding can slow the loss of hippocampal nerve cells,slow down the process of apoptosis,reduce the degree of seizure,and then play a role in brain protection.更多还原