【摘要】 目的建立一种简单、有效、稳定的离体蟾蜍心室颤动模型。方法 30只蟾蜍分为任氏液组、高钙组和高钙+Ad组,每组10只。采用斯氏法制备离体蛙心灌流标本。任氏液组离体蟾蜍心脏只灌流任氏液;高钙组和高钙+Ad组初始心室外容积固定为1.0mL,向任氏液组离体蟾蜍蛙心加入10%CaCl₂ 20μL即高钙组,高钙组离体蟾蜍蛙心出现心室颤动时,立刻加入0.1%盐酸肾上腺素20μL即高钙+Ad组。应用BL-420F生物机能实验系统记录离体蟾蜍蛙心心肌收缩曲线和心电图。结果高钙组与任氏液组比较心肌收缩力明显减小（P<0.01）,心肌收缩力曲线的基线明显上移,离体蟾蜍高钙时心电图检测可见高尖T波和正弦波,高钙组与任氏液组心率无明显变化（P>0.05）。高钙+Ad组与高钙组比较心肌收缩力基本恢复（P<0.01）,心肌收缩力曲线的基线逐渐下移,心率无明显变化（P>0.05）,心电图逐渐恢复至接近正常。结论高钙离体蟾蜍心室颤动模型成功建立,方法可靠稳定。更多还原

【Abstract】 Objective To construct a simple,effective and stable model of ventricular fibrillation of isolated toad heart.Methods A total of 30 toads were randomly divided into the Ringer′s solution group,the high-calcium group and the high-calcium+Ad group,with 10 for each.The perfusion specimens of isolated toad heart were fully prepared by Si′s method.The toad heart in the Ringer′s solution group was infused with Ringer′s solution only.The volume of the high-calcium group and the high-calcium+Ad group was 1.0 mL outside the ventricle.The Ringer′s solution group was infused with 10% CaCl₂ 20μL as the high-calcium group.When existing ventricular fibrillation,the high-calcium group was infused with 0.1% Epinephrine 20μL as the high-calcium+Ad group.BL-420 Fbiological and functional experimental system was applied to recorded the myocardial contraction curve and electrocardiogram.Results Myocardial contractility was significantly lower in the high-calcium group compared to the Ringer′s solution group（P<0.01）.The baseline of myocardial contraction curve significantly moved up in the high-calcium group with detection of high-point T wave and sine wave by electrocardiogram.There was no significant change in heart rate between the high-calcium group and the Ringer′s solution group（P>0.05）.Myocardial contractility of the high-calcium+Ad group basically restored,compared with that of the high-calcium group（P<0.01）,the baseline of myocardial contraction curve moved down and the heart rate did not change significantly（P>0.05）,then the electrocardiogram gradually returned to normal.Conclusion Model of ventricular fibrillation of isolated toad heart is constructed successfully,and the method is stable and credible.更多还原