【摘要】 为了解铜绿微囊藻附生细菌的多样性及其溶磷活性,采用平板稀释涂布法分离细菌,并利用16S rRNA基因序列分析确定其分类学地位,用钼锑抗比色法检测其溶磷活性,用苯磷酸二钠法检测其磷酸酶活性.结果表明:分离的细菌分属于4个门,即放线菌门、变形菌门、拟杆菌门和异常球菌-栖热菌门;其中83%以上的细菌至少对卵磷脂、植酸钙和磷酸钙中的1种具有溶磷活性,94%以上的细菌表现出1～3种磷酸酶活性,且导致磷培养基的pH值下降.铜绿微囊藻附生细菌中存在丰富的溶磷微生物,这些微生物对藻类获取足够的可溶性磷元素可能具有重要的意义.更多还原

【Abstract】 In order to understand the diversity and phosphate solubilization activity of Microcystis aeruginosa-associated bacteria,dilution plating procedure is used to isolate the bacteria,and the 16 S rRNA gene sequences are used to determine the taxonomic status of the bacteria,the molybdenum antimony resistance colorimetric and disodium phenyl phosphate methods are used to analyze solubilizing-phosphate activities and phosphatase activities of the bacteria,respectively.The results show that the isolates belong to four phylums,i.e.Actinobacteria,Proteobacteria,Bacteroidetes and Deinococcus-Thermus,respectively.And more than 83% of these bacteria exhibit solubilizing-phoshate activities at least on one of the three phosphorus sources such as lecithin,phytin and tricalcium phosphate,and more than 94% of these bacteria exhibit 1～3 kinds of phosphatases,and result in decline of the pH value of phosphorus media.There are abundant solubilizing-phosphate bacteria in the Microcystis aeruginosa-associated bacteria,and these microorganisms probably play an important role for the algae to obtain enough soluble phosphorus.更多还原