【摘要】 目的探讨不同浓度HU-308与纳米羟基磷灰石与聚己内酯（nHA/PCL）电纺丝膜对小鼠前成骨细胞MC3T3-E1生物学性能的影响。方法将静电纺丝膜制成圆形薄膜置于细胞培养板底部,使用含不同浓度HU-308(0、10^-10、10^-9、10^-8、10^-7、10^-6 mol/L)的培养液培养MC3T3-E1细胞;培养1、4、7 d,进行CCK-8检测增殖检测及碱性磷酸酶（ALP）活性的检测;Real Time-PCR检测培养7 d时细胞成骨相关基因OPG mRNA表达,观察培养24 h细胞骨架的形态。结果培育1、4、7 d后,与对照组相比,各实验组间细胞增殖活性增强,P<0.05;随着培育时间的延长,同一实验组细胞增殖活性逐渐增强,P<0.05。随着培育时间的延长,同一实验组MC3T3-E1细胞中的ALP活性增加,P<0.05。在相同时间点,与对照组比较,低浓度HU-308(10^-10 mol/L、10^-9 mol/L)促进OPG mRNA的表达,P<0.05;高浓度HU-308对OPG mRNA的表达起抑制作用,且浓度越高抑制作用越明显,P<0.05。成骨细胞随药物浓度的加大,细胞铺展面积有增大趋势且细胞骨架结构更加清晰,尤以10^-6 mol/L浓度组成骨细胞伸展范围最广。结论 HU-308和nHA/PCL电纺丝膜可促进MC3T3-E1细胞的黏附、增殖和分化。更多还原

【Abstract】 Objective To explore the effects of different concentrations of HU-308 and nHA/PCL electrospun membranes on the biological properties of mouse pre-osteoblasts（MC3 T3-E1）.Methods The round electrospun membranes were placed at the bottom of the cell culture plates. MC3 T3-E1 osteoblasts were inoculated and cultured in different concentrations of HU-308(0, 10^-10, 10^-9, 10^-8, 10^-7, 10^-6 mol/L). At 1, 4, and 7 days, cell proliferation and alkaline phosphatase（ALP） activity was detected; RT-qPCR was used to detect osteoblast-related genes OPG mRNA after cultured cells for 7 days. The morphology of the cytoskeleton after 24 h was observed.Results After incubation for 1, 4, and 7 days, compared with that in the control group, the proliferation activity of cells in each experimental group was enhanced, P<0.05; with the extension of incubation time, the proliferative activity of cells in the same experimental group increased gradually, P<0.05. With the extension of incubation time, ALP activity in MC3 T3-E1 cells in the same experimental group increased, P<0.05. At the same time point, compared with that in the control group, the low concentration of HU-308(10^-10 mol/L, 10^-9 mol/L) promoted the expression of OPG mRNA, P<0.05. High concentration of HU-308 inhibited the expression of OPG mRNA, and the higher the concentration was, the more significant the inhibition was, P<0.05. The results of phalloidine staining showed that the cells in the 10^-6 mol/L group had the largest spreading area and most explicit structure.Conclusion HU-308 and nHA/PCL electrospun membranes can promote the adhesion, proliferation, and differentiation of MC3 T3-E1 cells.更多还原