【摘要】 目的:利用超声辅助-离子液体微萃取-反相高效液相色谱测定银杏叶中咖啡酸、阿魏酸、芦丁、绿原酸、槲皮素、木犀草素、山柰酚、芹菜素、原儿茶酸有效成分。方法:采用RP-InertSustain C₁₈（150mm×4.6mm,5μm）色谱柱;流动相:甲醇（A）-0.2%乙酸溶液（B）;梯度洗脱;流速:0.5mL/min:检测波长:290nm;柱温:35℃;进样量20μL。结果:该条件下9种成分分离度良好,标准曲线呈线性关系,相关系数均≥0.9994（n=8）。加标回收率（n=3）平均值为98.40%～103.45%,相对标准偏差为0.75%～3.21%。结论:该方法简便快捷、灵敏度高、重复性好,不仅可较为全面地反映银杏叶中各成分含量,还可用于其质量控制研究。更多还原

【Abstract】 Objective: Simultaneous determination of caffeic acid, ferulic acid, rutin, chlorogenic acid, quercetin, luteolin, kaempferol, apigenin and protocatechuic acid in Ginkgo biloba L. by ultrasonic assisted-ionic liquid microextraction coupled with reversed-phase high performance liquid chromatography. Methods: RP-InertSustain C₁₈（150 mm×4.6 mm, 5μm） column was used; mobile phase was methanol（A）-0.2% acetic acid solution（B）; gradient elution; flow rate 0.5 mL/min: The detection was carried out at 290 nm; column temperature 35°C: injection volume 20μL. Results: The separation of the nine components was good under optimized conditions. The standard curve showed good linearity, and the correlation coefficient r≥0.9994（n=8）. The average recovery rate（n=3） was 98.40%～103.45%, and the RSD was 0.75%～3.21%. Conclusion: This method is simple and reliable, which not only provides a theoretical basis for quantitative detection of Ginkgo biloba L., but can be used for the quality control of Ginkgo biloba L..更多还原