【摘要】 目的:研究5-氮杂-2′-脱氧胞苷对HT22小鼠海马神经元细胞中NR2B 1472位点的酪氨酸磷酸化(p-Y1472 NR2B)的影响。方法:使用小鼠海马神经元细胞系HT22,对照组细胞用0.1%BSA处理,处理组细胞用10μmol/L 5-Aza-CdR处理。采用real time RT-PCR检测NR2B mRNA的表达;Western Blot检测NR2B和磷酸化NR2B-Y1472(p-Y1472 NR2B)的表达水平;MTS法检测细胞活力;流式细胞分析技术检测细胞周期。结果:HT22细胞在10μmol/L 5-Aza-CdR作用下,NR2B-Y1472的磷酸化水平升高,细胞活力下降,细胞周期受到阻滞。结论:5-Aza-CdR对小鼠行为记忆能力的影响可能与NR2B-Y1472磷酸化相关。更多还原

【Abstract】 Objective:To study the effects of 5-Aza-2’-deoxycytidine on the tyrosine phosphorylation of NR2 B 1472 site(p-Y1472 NR2 B) in the HT22 cells. Methods:The mice hippocampal neuron cell line HT22 was used in vitro experiment, cells in the control group were treated with 0.1 % BSA, and the treatment group were treated with 10 μmol/L 5-Aza-CdR. The mRNA level of NR2 B were measured by real time RT-PCR. NR2 B and p-Y1472 NR2 B were measured by Western Blot. Cell activity was detected by MTS method. Cell cycle was detected by flow cytometry. Results:In vitro experiments showed that 10 μmol/L 5-Aza-CdR can increased the phosphorylation level of NR2 B-Y1472, decreased the cell viability and arrested cell cycle. Conclusions:The effects of 5-Aza-CdR on behavioral memory ability may be related to the phosphorylation NR2 B-Y1472.更多还原