【摘要】 目的:检测食管鳞状细胞癌（esophageal squamous cell carcinoma,ESCC）中含WW结构域的氧化还原酶（WW domain-containing oxidoreductase,WWOX）蛋白和细胞外信号调节蛋白激酶1（extracellular signal regulated protein kinase 1,ERK1）表达及其与ESCC病人临床病理参数的相关性。方法:采用免疫组织化学EliVision^TM plus法检测150例ESCC和80例正常食管黏膜组织（对照组）中WWOX和ERK1蛋白的表达情况。结果:ESCC组织中WWOX和ERK1蛋白的阳性表达率分别为46.0%和58.7%;对照组中WWOX和ERK1蛋白的阳性率分别为85.0%和42.5%,差异均有统计学意义（P<0.05）。ESCC组织中WWOX蛋白阳性率与ERK1蛋白阳性率呈负相关关系（P<0.01）。WWOX和ERK1蛋白阳性表达率在ESCC病人不同肿瘤组织浸润深度、TNM分期和是否淋巴结转移间差异均有统计学意义（P<0.05～P<0.01）。生存分析显示,WWOX蛋白阳性表达病人的生存时间高于阴性者（P<0.05）。多因素回归分析显示,WWOX和ERK1蛋白阳性表达及TNM分期是影响ESCC病人术后生存的独立影响因子（P<0.05～P<0.01）。结论:WWOX和ERK1的异常表达参与了ESCC的发生、发展、浸润以及转移,早期联合检测WWOX和ERK1蛋白表达对ESCC的进展和预后判断有重要意义。更多还原

【Abstract】 Objective:To explore the expression levels of WW domain-containing oxidoreductase（WWOX） and extracellular signal regulated protein kinase 1（ERK1） in esophageal squamous cell carcinoma（ESCC）,and their correlations with the clinical characteristics.Methods:The expression levels of WWOX and ERK1 protein in 150 specimens of ESCC and 80 normal esophageal mucosa tissues were examined using EliVision^TM plus immunohistochemistry.Results:The positive rates of WWOX and ERK1 protein in ESCC tissues and normal esophageal tissues were 46.0% & 58.7% and 85.0% & 42.5%,respectively,and the differences of those between two groups were statistically significant（P<0.05）.The positive rate of WWOX protein expression was negatively correlated with the positive rate of ERK1 protein in ESCC tissues（P<0.01）.The differences of the positive expression rates of WWOX and ERK1 protein in ESCC patients with different tumor tissue invasion depth,TNM stage and lymph node metastasis were statistically significant（P<0.05 to P<0.01）.The results of survival analysis showed that the survival time of patients with positive WWOX expression was higher than that of patients with negative WWOX expression（P<0.05）.The results of multivariate regression analysis showed that the positive WWOX and ERK1 protein expression and TNM stage were the independent influencing factors of postoperative survival of ESCC patients（P<0.05 to P<0.01）.Conclusions:The abnormal expression of WWOX and ERK1 may be involved in the occurrence,progression and metastasis of ESCC.The combined detection of WWOX and ERK1 in early stage has an important role in predicting the progression and prognosis of ESCC.更多还原