【摘要】 目的:探讨N-乙酰半胱氨酸(NAC)对体外乙醇处理诱导的张氏肝细胞损伤的保护作用。方法:体外培养张氏肝细胞,实验分为对照组、乙醇处理组和NAC高、中、低剂量进行预处理的保护组,并测定NAC预处理对乙醇诱导的肝细胞损伤模型的细胞存活率,细胞内天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、活性氧(ROS)、细胞上清液中肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)水平的影响,评价NAC是否对乙醇造成的肝细胞损伤具有保护作用。结果:NAC预处理后,细胞的存活率高于乙醇处理组(P <0. 05),细胞形态学观察结果显示,NAC的干预组细胞形态优于乙醇处理组; NAC预处理显著降低细胞内AST、ALT、ROS和细胞上清液中TNF-α和IL-6的含量(P <0. 05)。结论:NAC干预可减轻体外乙醇处理诱导的肝细胞损伤,并降低细胞内ROS和炎性因子的水平。更多还原

【Abstract】 Objective: To investigate the protective effects of N-acetylcysteine( NAC) on ethanol-induced Chang hepatocellular injury.Methods: The Chang hepatocytes were cultured in vitro,and divided into the control group,ethanol treatment group,and high,medium and low dose NAC protection groups. The cell survival rate of ethanol-induced hepatocellulars injury model by NAC pretreatment was determined,and the effects of aspartate aminotransferase( AST),alanine aminotransferase( ALT),intracellular reactive oxygen species( ROS),tumor necrosis factor-α( TNF-α),and interleukin-6( IL-6) were evaluated. The protective effects of NAC on ethanol-induced Chang hepatocellular injury were analyzed. Results: After NAC pretreatment,the cell survival rate was higher than that of the ethanoltreated group( P < 0. 05). The results of cell morphology showed that the cellular morphology in NAC intervention group was better than that in ethanol-treated group. The NAC pretreatment could significantly reduce the contents of TNF-α and IL-6 in cell supernatant,and levels of AST,ALT and ROS in cells( P < 0. 05). Conclusions: The NAC intervention can alleviate the Chang hepatocellular injury induced by ethanol treatment in vitro,and reduce the levels of intracellular ROS and inflammatory factors.更多还原