【摘要】 目的探讨卵巢癌组织中转录激活因子-4（ATF-4）、第二个线粒体衍生的半胱氨酸蛋白酶激活剂（SMAC）蛋白的表达情况及临床意义。方法收集90例卵巢癌患者的卵巢癌组织标本和90例卵巢良性肿瘤患者的良性肿瘤组织标本。采用免疫组化染色法检测两种组织中ATF-4、SMAC蛋白的表达情况,并对卵巢癌组织中ATF-4、SMAC蛋白表达与卵巢癌患者病理特征的关系进行分析。结果免疫组化染色结果显示,卵巢癌组织中ATF-4的阳性表达率明显高于卵巢良性肿瘤组织（P﹤0.01）。卵巢癌组织中SMAC蛋白的阳性表达率明显低于卵巢良性肿瘤组织（P﹤0.01）。FIGO分期为Ⅲ～Ⅳ期、有淋巴结转移、组织学分级为G3级的上皮性卵巢癌患者上皮性卵巢癌组织中ATF-4蛋白的阳性表达率均高于FIGO分期为Ⅰ～Ⅱ期、无淋巴结转移、组织学分级为G₁～G₂级的患者（P﹤0.05）;FIGO分期为Ⅲ～Ⅳ期、有淋巴结转移、组织学分级为G₃级的上皮性卵巢癌患者上皮性卵巢癌组织中SMAC蛋白的阳性表达率均明显低于FIGO分期为Ⅰ～Ⅱ期、无淋巴结转移、组织学分级为G₁～G₂级的患者（P﹤0.01）;不同病灶直径的上皮性卵巢癌组织中ATF-4、SMAC蛋白的阳性表达率比较,差异均无统计学意义（P﹥0.05）。结论上皮性卵巢癌组织中ATF-4蛋白的表达上调,SMAC蛋白的表达下调,且ATF-4、SMAC蛋白表达可能与上皮性卵巢癌患者的病情进展有一定关系。更多还原

【Abstract】 Objective To investigate the expression of activating transcriptional factor-4（ATF-4） and second mitochondrial-derived activator of caspase（SMAC） protein in ovarian cancer tissues and to determine the clinical significance.Method The ovarian cancer tissues and benign ovarian tumor specimens from 90 cases with ovarian cancer and90 patients with benign ovarian tumors were collected,in which the expression of ATF-4 and SMAC proteins were detected by immunohistochemical staining,furthermore,the relationship between expression of ATF-4 and SMAC proteins and the pathological characteristics of ovarian cancer patients were analyzed.Result The positive expression rate of ATF-4 protein in epithelial ovarian cancer tissues were significantly higher than those in benign tumor tissues（P<0.01） as indicated by immunohistochemical staining.Besides,SMAC protein expression was evidently decreased compared to benign tumor tissues（P<0.01）.The positive expression rates of ATF-4 protein in the specimens with FIGO stage Ⅲ-Ⅳ,presence of lymph node metastasis,histological classification of G3 in epithelial ovarian cancer tissues were significantly higher compared to those with FIGO stage Ⅰ-Ⅱ,negative lymph node metastasis and histological classification of G₁-G₂（P<0.05）;as to SMAC protein expression,FIGO stage Ⅲ-Ⅳ,presence of lymph node metastasis,histological classification of G3 in epithelial ovarian cancer tissues were associated with markedly decreased positive expression of SMAC protein compared to those with FIGO stage Ⅰ-Ⅱ,negative lymph node metastasis,histological classification of G₁-G₂（P<0.01）;the positive expression rates of ATF-4 and SMAC proteins in epithelial ovarian cancer tissues of varying diameter were comparable（P>0.05）.Conclusion Up-regulation of ATF-4 protein expression and down-regulation of SMAC protein expression in epithelial ovarian cancer tissues may be implicated with the development of epithelial ovarian cancer.更多还原