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NaN3对香蕉农科1号不定芽诱变的影响
Effect on Mutation of Adventitious Buds of Banana Nongke 1 with Sodium Azide
【摘要】 研究了不同NaN3浓度和诱变时间处理对香蕉品种农科1号不定芽的诱变效果。结果表明,0.15 g/L NaN3处理3 h和0.25 g/L NaN3处理2h为农科1号不定芽诱变的适宜处理组合,在这2个诱变条件下,农科1号香蕉不定芽致死率接近半致死率,存活的不定芽能够恢复生长和增殖,容易获得再生植株。
【Abstract】 Different concentrations of sodium azide combined with different mutation times were applied to mutagenize the adventitious buds of Nongke 1 banana. The results showed that both 0.15 g/L sodium azide combined with 3 h treatment and 0.25 g/L sodium azide combined with 2 h treatment were suitable combinations for mutation of adventitious buds of Nongke 1 banana, with which the lethal rate was close to 50%, the survival buds were able to restored growth and multiplication after the mutation.
【关键词】 NaN3;
香蕉;
农科1号;
不定芽;
诱变;
【Key words】 sodium azide; banana; Nongke 1; adventitious bud; mutation;
【Key words】 sodium azide; banana; Nongke 1; adventitious bud; mutation;
【基金】 广东省科技厅农业攻关项目(2011B020410004)
- 【文献出处】 现代农业科技 ,Modern Agricultural Science and Technology , 编辑部邮箱 ,2020年14期
- 【分类号】S436.68
- 【下载频次】78