【摘要】 目的研究过氧化氢酶基因在腰椎管狭窄症(LSCS)病人肥大黄韧带标本中的表达。方法黄韧带标本通过外科手术获自2013年1月至2015年12月间柳州市柳铁中心医院75例椎管狭窄病人(LSCS组)和75例腰椎间盘突出症病人(LDH组)。使用卡尺在最厚点测量黄韧带厚度至0.01 mm的精度。黄韧带弹性蛋白降解和纤维化的程度分别通过苏木精和伊红染色和Masson三色染色分级(0～4级)。比较两组之间的黄韧带测量值,组织学数据和免疫组织学结果。结果 LSCS组的平均黄韧带厚度显著高于LDH组(分别为5.99 mm和2.95 mm,P=0.004)。脊柱狭窄样本中弹性蛋白降解和黄韧带纤维化明显比椎间盘突出样本严重[(3.04±0.50)比(0.79±0.60),P=0.007;(3.01±0.47)比(0.66±0.42),P=0.009)]。结论过氧化氢酶的表达降低与LSCS黄韧带肥大有关。更多还原

【Abstract】 Objective To study the expression of catalase gene in the ligamentum flavum fatty(LF)of patients with lumbar spinal stenosis(LSCs).Methods The ligamentum flavum specimens were obtained from 75 patients with spinal stenosis(LSCs group)and 75 patients with lumbar disc herniation(LDH group).Measure the thickness of the ligamentum flavum to an accuracy of 0.01 mm at the thickest point with a caliper.The degradation and fibrosis of elastin in ligamentum flavum were graded by hematoxylin and eosin staining and Masson trichrome staining(0-4 grade).The results of measurement,histology and immunohistochemistry were compared between the two groups.Results The average LF thickness was significantly higher in the LSCS group than in the LDH group(5.99 mm vs.2.95 mm,P=0.004). Elastin degradation(3.04±0.50 vs.0.79±0.60,P=0.007)and fibrosis(3.01±0.47 vs.0.66±0.42,P=0.009)of the LF were significantly more severe in spinal stenosis samples than in the disc herniation samples.Conclusion The decreased expression of catalase is associated with LF hypertrophy in patients with LSCS.更多还原