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赣南脐橙致腐菌的分离鉴定及其对2-苯乙醇敏感性分析

Isolation and Identification of Citrus Pathogens from Navel Orange in Southern Jiangxi Province and Sensitivity Analysis to 2-Phenylethanol Alcohol

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【作者】 熊大维; 顾斌涛; 刘兰; 黄筱萍;

【Author】 XIONG Da-wei;GU Bin-tao;LIU Lan;Institution of Microbiology, Jiangxi Academy of Sciences;

【通讯作者】 黄筱萍;

【机构】 江西省科学院微生物研究所;

【摘要】 从采摘后贮存自然发病的赣南脐橙中分离出4种病原真菌,根据菌株形态学观察和rDNA-ITS序列分析进行鉴定,这4种病原真菌分别为意大利青霉(Penicillium italicum)、指状青霉(Penicillium digitatum)、柑橘链格孢(Alternaria citri)和芽枝状枝孢(Cladosporium cladosporioides)。生物保鲜剂2-苯乙醇(2-PE)可有效地抑制这4种病原真菌的孢子萌发和菌丝生长,但对不同致腐菌抑菌效果差异显著(P<0.05)。2-PE对芽枝状枝孢霉、指状青霉菌、柑橘链格孢和意大利青霉菌菌丝生长的最小抑菌浓度分别为1.4、1.8、2.2和2.4 g/L。

【Abstract】 Four citrus pathogens were isolated from rotten orange of Southern Jiangxi Province after harvesting. According to the observation of the morphological characteristics of fungi and the analysis of rDNA-ITS sequence, the four citrus pathogens were identified as Penicillium italicum, Penicillium digitatum, Alternaria citri and Cladosporium cladosporioides. The biopreservative 2-phenylethyl alcohol can effectively inhibit the spore germination and hyphae growth of the four citrus pathogens, but the antibacterial effects of different saprophytic fungi were significantly different(P<0.05). The minimum inhibitory concentrations for the hyphae growth of C. cladosporioides, P. digitatum, A. citri and P. italicum were 1.4, 1.8, 2.2 and 2.4 g/L, respectively.

【基金】 江西省重点研发项目(S2018ZPYFE1016);江西省科学院重大研究专项(2018-YZD1-03)
  • 【文献出处】 安徽农业科学 ,Journal of Anhui Agricultural Sciences , 编辑部邮箱 ,2020年07期
  • 【分类号】S436.66
  • 【被引频次】3
  • 【下载频次】242
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