【摘要】 建立了食用植物油中5种儿茶素类抗氧化剂的HPLC检测方法。样品经正己烷溶解,再用饱和正己烷的乙腈萃取,振荡,超声25 min,离心后取乙腈层,定容,过0. 45μm滤膜。然后采用Waters XTerra RP-C18色谱柱(4. 6 mm×250 mm,5μm),选择甲醇-水(体积比1∶9)+乙腈作为流动相,流速为1 mL/min,梯度洗脱。综合分析各化合物吸收光谱,选择216、230、280 nm作为检测波长。结果表明:建立的HPLC检测橄榄油中5种儿茶素类化合物的检测方法,化合物分离度好,峰型尖锐,加标回收率在80%～101. 3%之间;方法通用性和精密度实验数据均表明该方法满足检测需要。更多还原

【Abstract】 A method for determination of five natural catechins antioxidants in edible vegetable oil by HPLC was established. The samples were dissolved in n-hexane,then extracted with saturated n-hexane acetonitrile for 25 min under the conditions of oscillation and ultrasound,then centrifuged and the acetonitrile layer was taken. The acetonitrile layer was filtered with 0. 45 μm membrane after constant volume. The detection conditions were obtained as follows: Waters XTerra RP-C18 chromatographic column(4. 6 mm × 250 mm,5 μm),methanol-water(volume ratio 1∶ 9) and acetonitrile as the flow phase,flow rate 1 mL/min,gradient elution,and detection wavelength 216,230,280 nm. The results showed that the method establised was used to detect five kinds of catechins in olive oil,and the separation degree was good,the peak absorption was sharp,and the recovery rate of standard addition was between 80% and 101. 3%. All data of method universality and precision test illustrated that the method was able to meet test needs very well.更多还原