【摘要】 目的探讨生成像(BLI)和MRI相结合对移植入大鼠损伤肝脏内的间充质干细胞(MSCs)的实时、无创示踪的能力及移植的MSCs对大鼠损伤肝脏的功能修复作用。方法对急性肝损伤大鼠模型移植标记了荧光素酶和SPION的MSCs,分析大鼠的血浆转氨酶水平及肝脏病理学改变,观察MSCs对肝功能损伤的修复作用,并利用BLI和MRI观察MSCs在大鼠肝脏内积累的时间进程,最后进行大鼠肝脏组织切片的原位免疫组织化学染色。结果 MSCs移植后的第5、9、10天检测的大鼠血浆天冬氨酸转氨酶和丙氨酸转氨酶水平较对照组显著降低(P均<0.05)。MSCs移植后第1天,大鼠肝脏区域的生物发光强度显著增强,T2~*WI标准化信号强度明显减低,于移植后第10天才逐渐恢复到移植前水平。并且随着大鼠肝脏生物发光强度的逐渐下降,荧光素酶阳性的MSCs数目亦随时间进程逐渐减少。结论移植MSCs对大鼠损伤肝脏的功能具有良好的修复作用;且将BLI和MRI相结合可实时、无创示踪移植入大鼠损伤肝脏内的MSCs。更多还原

【Abstract】 Objective To explore the ability of combining bioluminescence imaging(BLI) and MRI to make real-time and non-invasive tracer of mesenchymal stem cells(MSCs) transplanted into damaged liver of rats; and to study the functional repair effect of transplanted MSCs on injured liver in rats. Methods Rat model with acute liver injury were transplanted the MSCs of labeled luciferase and SPION. Rat plasma aminotransferase levels and liver pathology change were analyzed, and the repair effect of MSCs on liver function injury were observed. The time course of MSCs accumulation in rat liver was observed by BLI and MRI. In situ immunohistochemical staining of rat liver tissue sections was performed. Results Plasma aspartate aminotransferase and alanine aminotransferase levels were significantly lower on days 5, 9 and 10 after transplantation than in the control group(all P<0.05). On the first day after the transplantation of MSCs, the bioluminescence intensity in the liver area of rats was significantly enhanced, and the T2~*WI standardized signal intensity was significantly reduced, and then gradually returned to the pre-transplantation level 10 days after the transplantation. The number of luciferase-positive MSCs decreased with the decrease of bioluminescence intensity. Conclusion Transplantation of MSCs has a good repair effect on the damaged liver function of rats. In addition, BLI combined with MRI is used for real-time and non-invasive tracing of MSCs transplanted into the injured liver of rats.更多还原