【摘要】 目的:探究塞来昔布（CXB）对转化生长因子-β1（TGF-β1）诱导人胃癌细胞SGC-7901上皮细胞-间充质转化（EMT）过程的影响及其分子机制。方法:用10 ng·ml^-1TGF-β1诱导SGC-7901细胞建立EMT模型,将建模成功的胃癌细胞分为5组:模型组、白藜芦醇组(Res,50μmol·L^-1)、CXB低(40μmol·L^-1)、高(100μmol·L^-1)剂量组、Res+CXB组(50μmol·L^-1+100μmol·L^-1),以未建模的SGC-7901细胞作为对照组,采用实时荧光定量PCR（qRT-PCR）检测SGC-7901中sirtuin 1 mRNA表达情况,分别采用MTT实验、划痕实验和transwell小室检测SGC-7901细胞增殖、迁移和侵袭情况,蛋白免疫印迹（Western Blot）检测SGC-7901细胞中钙粘附蛋白-E（E-cadherin）、波形蛋白（vimentin）表达情况。结果:模型组SGC-7901细胞呈现梭形和纺锤形,且细胞间隙变大、排列松散,提示造模成功。模型组和Res组SGC-7901细胞中sirtuin 1 mRNA表达水平、SGC-7901细胞增殖率、迁移能力、侵袭能力及vimentin表达水平显著高于对照组,E-cadherin表达水平显著低于对照组（P<0.05）;与模型组相比,CXB低、高剂量组SGC-7901细胞中sirtuin 1 mRNA表达水平、SGC-7901细胞增殖率、迁移能力、侵袭能力及vimentin表达水平显著降低,E-cadherin表达水平显著升高（P<0.05）;Res+CXB组SGC-7901细胞中sirtuin 1 mRNA表达水平、SGC-7901细胞增殖率、迁移能力、侵袭能力vimentin表达水平显著低于Res组,E-cadherin表达水平显著高于Res组（P<0.05）。结论:CXB能够下调SGC-7901细胞中sirtuin 1表达,进而抑制TGF-β1诱导的人胃癌细胞SGC-7901 EMT过程。更多还原

【Abstract】 Objective:To investigate the effect of celecoxib（CXB）on SGC-7901 epithelial-mesenchymal transition（EMT）process induced by TGF-β1 in human gastric cancer cells and its molecular mechanism.Methods:SGC-7901 cells were induced by 10ng·ml^-1TGF-β1 to establish EMT model,and the gastric cancer cells were divided into five groups,named the model group,resveratol group(Res,50μmol·L^-1),CXB low dose(40μmol·L^-1)group,CXB high dose(100μmol·L^-1)group,Res+CXB group(50μmol·L^-1+100μmol·L^-1),and the SGC-7901 cells without modeling were used as the control group.Real-time quantitative PCR（qRT-PCR）was used to detect SGC-7901 sirtuin 1 mRNA,and the cell proliferation,migration and invasion was detected by MTT assay,scratch assay and Transwell assay,respectively,the expressions of vimentin and E-cadherin were detected by Western blot.Results:The SGC-7901 cells in the model group showed shuttle and spindle shapes,the intercellular space became larger,and the cells became loosely arranged,which indicated that the modeling was successful.The expression level of sirtuin 1 mRNA,the proliferation,migration,invasion and vimentin expression of SGC-7901 cells in the model group and Res group were significantly higher than those in the control group,and the expression level of E-cadherin in SGC-7901 cells was significantly lower than that in the control group（P<0.05）.Compared with those in the model group,the expression level of sirtuin 1 mRNA,the proliferation,migration,invasion and vimentin expression of SGC-7901 cells was significantly decreased,and the expression level of E-cadherin in SGC-7901 cells was significantly increased（P<0.05）.The expression level of sirtuin 1 mRNA,the proliferation,migration,invasion and vimentin expression of SGC-7901 cells in Res+CXB group were significantly lower than those in Res group,and the expression level of E-cadherin was significantly higher than that in Res group（P<0.05）.Conclusion:CXB can down-regulate SGC-7901 cells sirtuin 1 expression,and inhibit SGC-7901 EMT process induced by TGF-β1 in human gastric cancer cell.更多还原