【摘要】 目的:研究p-香豆酸（p-CA）对急性缺氧引发的小鼠脑水肿的预防作用。方法:45只健康雄性ICR小鼠随机分为正常组、模型组、阳性对照组(25 mg·kg^-1·d^-1人参皂苷Rg1)、p-CA低剂量组(25 mg·kg^-1·d^-1)和p-CA高剂量组(100 mg·kg^-1·d^-1),连续灌胃（ig）给药7 d后急性缺氧（9. 5%O₂）处理6 h。检测小鼠脑组织含水量、抗氧化能力（T-AOC,SOD,CAT和MDA）、ATP的含量及无氧代谢酶（LDH,PK和PFK）和Na⁺,K⁺-ATP酶活力,并观察脑组织的苏木精-伊红（HE）染色切片。结果:与正常组相比,急性缺氧6 h后小鼠脑组织含水量显著增加2. 63%（P <0. 05）,而高剂量的p-CA干预能有效抑制该指标的上升（P <0. 05）,与HE染色切片观察结果一致;同时,高剂量的p-CA能显著提高小鼠脑组织抗氧化能力（T-AOC↑,SOD↑,CAT↑和MDA↓）和Na⁺,K⁺-ATP酶活力（P <0. 05）。结论:p-CA对小鼠缺氧性脑水肿具有较好的预防效果,其作用机制可能与提升急性缺氧下脑组织抗氧化能力和Na⁺,K⁺-ATP酶活力有关。更多还原

【Abstract】 Objective: To investigate the effects of p-coumalic acid（ p-CA） on preventing hypoxia-induced cerebral edema in mice. Methods: Forty-five healthy male ICR mice were randomly divided into control group,model group,positive group( 25 mg·kg^-1·d^-1 ginsenoside Rg1),p-CA low dose group( 25 mg·kg^-1·d^-1) and p-CA high dose group( 100 mg·kg·d^-1). After being orally administrated with the test materials for 7 d,all the mice except control group were underwent 9. 5% O₂ for 6 h. The cerebral water content,anti-oxidative indexes（ T-AOC,SOD,CAT activities and MDA content）,ATP content,anaerobic metabolism-related enzymes（ LDH,PK and PFK activities）,Na⁺,K⁺-ATPase activity and HE（ Hematoxylin-eosin） staining were detected. Results:The cerebral water contents increased significantly by 2. 63% in the mice treated with acute hypoxia（ 9. 5% O₂）for 6 h（ P < 0. 05） as compared with the normal group,and administration with high-dose p-CA for 7 d could inhibit significantly this index（ P < 0. 05）. These results were confirmed by the HE staining. Moreover,high-dose p-CAcould significantly increase the antioxidant activities（ T-AOC ↑,SOD ↑,CAT ↑ and MDA ↓） and Na⁺,K⁺-ATPase activity of brain（ P < 0. 05）. Conclusion: p-CA has great preventive effect on hypoxia-induced cerebral edema in mice. The action mechanisms of p-CA could be due to its abilities of improving antioxidant capacity and enhancing the activity of Na⁺,K⁺-ATPase in brain under acute hypoxia.更多还原