【摘要】 目的:本实验探讨海松子提取物(EKps)对阿尔茨海默病(AD)转基因秀丽隐杆线虫体内β-淀粉样蛋白(β-amyloid,Aβ)毒性的影响及作用机制。方法:线虫设空白对照组、EKps组和EGb761阳性对照组。利用瘫痪实验比较各组AD线虫的Aβ毒性,硫磺素s染色测定各组AD线虫体内Aβ表达量的变化,胡桃醌诱导的氧化应激实验分析线虫的抗氧化应激能力,用标记谷胱甘肽s转移酶-4(GST-4::GFP)的线虫观察线虫体内GST-4表达量。结果:较之对照组,EKps延缓了由Aβ毒性导致的AD线虫瘫痪(P <0. 000 1),减少了AD线虫体内Aβ的沉积(P <0. 000 1),也增强了胡桃醌诱导下线虫的抗氧化应激能力(P=0. 001),并增加了线虫体内GST-4的表达(P <0. 000 1)。结论:EKps具有减少Aβ聚集从而减轻AD线虫体内Aβ毒性的作用,机制可能与增强线虫的抗氧化应激能力相关。更多还原

【Abstract】 Objective: This study aimed to discuss the effect of the extract of korean pine seed (EKps) on the toxicity of β-amyloid (Aβ) in an Alzheimer’s disease (AD) model of Caenorhabditis elegans (C. elegans) and explore its mechanism. Methods: A blank control group,EKps group and EGb761 positive control group were set.The Aβ toxicity in the AD C. elegans was compared by paralysis test. Changes of Aβ expression in the AD C. elegans in each group were measured by thioflavin-s staining. The oxidative stress experiment was induced by juglone to observe whether EKps can enhance the ability of anti-oxidative stress. C. elegans labeled with glutahtione s transferase-4 (GST-4: : GFP) were used for determining the expression of GST-4. Results: Aβ-induced paralysis in the AD C. elegans is alleviated by EKps (P < 0. 000 1). EKps also reduced the desposits of Aβ in the AD C. elegans (P < 0. 000 1) and enhanced the ability of anti-oxidative stress (P = 0. 001). In addition,EKps increased the expression of GST-4 in C. elegans (P < 0. 000 1). Conclusion: EKps alleviated the toxicity of Aβ in the AD C. elegans through reducing the deposition of Aβ,which may be related to enhancing the ability of C. elegans to resist oxidative stress.更多还原