【摘要】 目的检测被转化因子激活的长链非编码RNA(LncRNA-ATB)在非小细胞肺癌(NSCLC)细胞中的表达,及沉默Lnc RNA-ATB对NSCLC细胞侵袭和转移能力的影响。方法采用实时荧光定量聚合酶链反应(qRT-PCR)检测NSCLC细胞和正常支气管上皮细胞(HBEC)中LncRNA-ATB的表达水平;采用细胞划痕实验和Transwell实验检测沉默Lnc RNA-ATB对NSCLC细胞侵袭和转移的影响;采用荧光素酶报告基因实验和qRT-PCR检测LncRNA-ATB与microRNA-141(miR-141)的调控关系。结果LncRNA-ATB在NSCLC细胞中的表达高于HBEC细胞(P<0.05)。沉默NSCLC中LncRNAATB表达后,细胞侵袭和转移能力降低。LncRNA-ATB可竞争性结合miR-141,并下调其表达。结论LncRNA-ATB在NSCLC中表达上调,并可通过竞争性结合miR-141,促进NSCLC的侵袭和转移。更多还原

【Abstract】 Objective To explore the expression of LncRNA-ATB in non-small cell lung cancer(NSCLC), and investigate the effect and underlying mechanism of LncRNA-ATB on migration and invasion of NSCLC cells. Methods Expression of LncRNA-ATB in NSCLC cells and normal bronchial epithelial cells was analyzed by qRT-PCR. Effect of LncRNA-ATB on migration and invasion of NSCLC cells was detected by Transwell assay and wound healing assay. Luciferase assay was performed to detect whether LncRNA-ATB could sequester miR-141. qRT-PCR was performed to detect the relationship between LncRNA-ATB and miR-141 in NSCLC cells. Results LncRNA-ATB was up-regulated in the NSCLC cells compared to the normal bronchial epithelial cells(P < 0.05). Migration and invasion of NSCLC cells were suppressed after silencing LncRNA-ATB. LncRNA-ATB could directly bind to miR-141 and down-regulate its expression in NSCLC cells. Conclusions LncRNA-ATB is upregulated in NSCLC cells, and could promote migration and invasion of NSCLC cells by competitive binding to miR-141.更多还原