【摘要】 番茄斑萎病毒Tomato spotted wilt tospovirus(TSWV)是严重危害世界经济作物的一种病毒,寄主范围广泛。我们在研究中发现番茄斑萎病毒能侵染我国大蒜Allium sativum L.。利用摩擦接种法将TSWV接种到健康大蒜上,结果显示:接种14 d后大蒜新生叶片出现褪绿和白色斑点症状。ELISA检测显示大蒜叶片汁液与TSWV的单克隆抗体产生血清学反应,采用TSWV N基因的引物对大蒜叶片总RNA进行RT-PCR,结果扩增出约800 bp的条带,在NCBI上BLAST显示与TSWV YN5576的同源性最高,为98.52%。这些数据表明番茄斑萎病毒系统侵染我国大蒜。更多还原

【Abstract】 Tomato spotted wilt tospovirus(TSWV) causes severe damages to economic crops worldwide, and it can infect a wide range of plant hosts. To determine whether TSWV infect Chinese garlic, the extracts of Nicotiana benthamiana leaves that were infected with TSWV were rub-inoculated to garlic plants. Garlic plants showed chlorotic and white spot symptoms on new leaves at 14 d post inoculation. Then the garlic leaves were detected by ELISA and RT-PCR.Positive results were obtained in the Dot-ELISA of the garlic leaf extracts with monoclonal antibody of TSWV. Furthermore, the target fragments of 800 bp were obtained from all infected garlic leaves by RT-PCR using the specific primers for N gene of TSWV. The sequence of the RT-PCR products from infected garlic leave was analyzed at NCBI, and the results showed that it shared 98.52% of nucleic acids with TSWV YN5576 isolate. Therefore, our results showed that Chinese garlic could be infected by TSWV.更多还原