【摘要】 目的:探讨老年带状疱疹后神经痛(postherpetic neuralgia, PHN)病人外周血中Th17、Treg细胞及相关细胞因子的表达及其临床意义。方法:采用流式细胞术检测60例老年带状疱疹后神经痛病人及30例健康对照者外周血中Th17、Treg细胞水平,并计算Th17/Treg比率,ELISA方法检测外周血中白介素-6 (Interleukin-6, IL-6)、白介素-10 (Interleukin-10, IL-10)、白介素-17 (Interleukin-17,IL-17)及人肿瘤坏死因子-α(TNF-α)的表达水平。结果:与对照组相比,病例组病人外周血Th17的含量及IL-6、IL-17表达水平显著降低(P <0.05);Treg细胞含量及IL-10、TNF-α表达水平显著升高(P <0.05),且Th17/Treg比率较对照组降低,差异具有统计学意义(P <0.05)。结论:Th17细胞在PHN外周血中显著降低,Treg细胞显著增高,与二者相关的细胞因子也相应变化,Th17/Treg比率显著降低,提示两者通过多种机制参与机体的免疫应答,Th17/Treg的失衡可能在老年PHN的发病机制中起重要作用。更多还原

【Abstract】 Objective: To study the changes and clinical significance of Th17 and Treg cells as well as their associated cytokine expression in peripheral blood of elderly patients with postherpetic neuralgia(PHN).Methods: Flow cytometry and ELSIA assay were used to detect the expression ratio of Th17 and Treg cells and the serum levels of IL-6, IL-10,IL-17 and TNF-α in peripheral blood of 30 healthy individuals and 60 elderly patients with PHN. Then we analyzed the differences of the above detection index between healthy subjects and elderly patients with PHN. Results: The expression ratio of Th17 cells in peripheral blood of elderly patients with PHN and the serum levels of IL-6、IL-17 were significantly lower than those in healthy subjects(P < 0.05).The expression ratio of Treg cells in peripheral blood of elderly patients with PHN was significantly higher than that in healthy subjects(P < 0.05),The ratio of Th17/Treg in elderly patients with PHN was significantly lower than that in healthy subjects(P < 0.05); the serum levels of IL-10 and TNF-α in peripheral blood of elderly patients with PHN was significantly higher than that in healthy subjects(P < 0.05). Conclusion: Th17 and Treg cells and their related cytokines may be involved in the process of PHN in elderly patients and may have a great significance on the treatment and prevention of elderly patients with PHN.更多还原