【摘要】 肠黏膜微血管内皮细胞在肠道中广泛存在,为研究流感病毒唾液酸α2,3半乳糖(SAα2,3Gal)和α2,6半乳糖(SAα2,6Gal)两种受体在肠黏膜微血管内皮细胞表面的表达情况,本试验利用植物凝集素免疫荧光染色方法和流式细胞术分别进行了定性和定量分析。结果显示,大鼠肠黏膜微血管内皮细胞(RIM-MVECs)表面同时表达唾液酸α2,3-半乳糖和α2,6-半乳糖两种受体,即同时表达人流感病毒受体和禽流感病毒受体,并且唾液酸α2,6半乳糖的表达量显著高于α2,3半乳糖受体的表达量。该试验结果表明,RIM-MVECs可以被人源和禽源流感病毒感染,且更易被人源流感病毒感染。更多还原

【Abstract】 Intestinal mucosa microvascular endothelial cells are widely distributed in the intestinal tract. To study the expression of influenza virus sialic acid receptor on the surface of intestinal mucosa microvascular endothelial cells, immuno?uorescent staining and flow cytometry were used. The results showed that RIM-MVECs expressed α-2, 3-and α-2, 6-linked sialic acid receptors and the expression of SAa2,6 Gal receptor on RIM-MVECs was higher than that of SAa2,3 Gal receptor, suggesting that RIM-MVECs may be more susceptible to human influenza virus infection.更多还原