【摘要】 为了确定河南省某猪场导致保育仔猪死亡的病原菌,从病死猪的肺脏、肝脏和脾脏分离得到一株优势菌。结果显示,经16S rDNA测序确定该优势菌为杀鲑气单胞菌;动物致病试验显示,分离菌株对小鼠具有致病性;药敏试验显示,该分离菌对磺胺异噁唑、四环素、土霉素、阿莫西林/克拉维酸、卡那霉素、氨苄西林等耐药;毒力基因PCR检测显示,act、ast、fla、lipase毒力基因为阳性,alt、elastase为阴性。本研究首次从发病猪体内分离到杀鲑气单胞菌,并为该条件致病菌的诊疗提供参考依据。更多还原

【Abstract】 To investigate the pathogenic bacteria which caused the piglets dead at a farm located in Henan province, a strain of the dominant bacterium was isolated from lungs, heart and liver of a dead piglet. The isolate was identified as Aeromonas salmonicida by 16 S rDNA PCR amplification and sequencing. Animal pathogenicity test showed that the isolate had pathogenicity to mice. The result of Antimicrobial susceptibility test by the disc diffusion method indicated that the isolate was resistant to sulfamethoxazole, tetracycline, oxytetracycline, amoxicillin-clavulantic acid, kanamycin, ampicillin and so on.This study could provide the reference basis for clinical treatment of the conditional pathogen.更多还原