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板蓝根注射液的HPLC特征图谱研究

HPLC Characteristics Spectrum of Banlangen Injection Research

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【作者】 王亚芳; 段文龙; 周艳飞; 李浛; 张连彦; 钟昆芮;

【Author】 WANG Ya-fang;DUAN Wen-long;ZHOU Yan-fei;LI Han;ZHANG Lian-yan;ZHONG Kun-rui;Beijing Veterinary Drug Monitoring Institute;China Institute of Veterinary Drug Control;

【通讯作者】 周艳飞;

【机构】 北京市兽药监察所; 中国兽医药品监察所;

【摘要】 建立了板蓝根注射液HPLC特征图谱。采用Waters Atlantis T3(5μm,4.6×250 mm)色谱柱,以甲醇为流动相A,水为流动相B,进行梯度洗脱;流速为0.8 mL/min;柱温为30℃;检测波长250 nm。板蓝根注射液的色谱图基本相同,检出特征共有峰有9个。该方法简便,灵敏度高,重复性好,结果准确可靠,可作为板蓝根注射液有效的鉴别和质量评价方法。

【Abstract】 To establish HPLC profile of Banlangen injection, the Waters Atlantis T3(5 μm, 4. 6 x 250 mm)column was used. With methanol as mobile phase A and water as mobile phase B, gradient elution was/min; the column temperature was 30 ℃; the detection wavelength was 250 nm. The chromatograms of Banlangen injection were basically the same. There are 9 common peaks for the detected features. This method is simple, sensitive and reproducible. The results are accurate and reliable, and can be used as an effective method for identification and quality evaluation of Banlangen injection.

【基金】 科技部国家重点研发计划专项(2017YFD0501503)
  • 【文献出处】 中国兽药杂志 ,Chinese Journal of Veterinary Drug , 编辑部邮箱 ,2019年12期
  • 【分类号】S853.7
  • 【下载频次】144
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