【摘要】 【目的】探讨肉桂醛对子宫内膜癌RL95-2细胞凋亡的影响及其作用机制。【方法】选取肉桂醛作用子宫内膜癌RL95-2细胞,噻唑蓝(MTT)比色法检测子宫内膜癌RL95-2细胞的增殖活性和肉桂醛的IC50,Hoechst 33258染色荧光显微镜观察细胞凋亡形态学改变,流式细胞术检测RL95-2细胞的凋亡百分率,Western blot检测肉桂醛对RL95-2细胞Cleaved caspase-3、Caspase-3、NF-κB、IL-6和IGF-R蛋白表达的影响。【结果】肉桂醛可降低子宫内膜癌RL95-2细胞的增殖率,与药物浓度和作用时间有关;与溶剂对照组比较,肉桂醛组(0.29、0.59和1.20 mg/mL)作用48 h后RL95-2细胞的凋亡百分率明显增高(P <0.01),出现典型的凋亡小体,Cleaved caspase-3蛋白的表达明显增加(P <0.01),Caspase-3蛋白的表达无明显变化(P> 0.05),而NF-κBp65、IL-6和IGF-R蛋白的表达均明显降低(P <0.05)。【结论】肉桂醛可降低RL95-2细胞NF-κB·p65、IL-6和IGF-R蛋白的表达,促进RL95-2细胞的凋亡,从而起到抗子宫内膜癌作用。更多还原

【Abstract】 【Objective】To investigate the effect of cinnamaldehyde on the apoptosis of RL95-2 cell in endometrial carcinoma.【Methods】Endometrial carcinoma RL95-2 cells were treated with cinnamaldehyde,and the proliferation activity and IC50 of endometrial carcinoma RL95-2 cells were detected by MTT colorimetry assay. Apoptotic morphology was observed after Hoechst 33258 staining. The percentage of apoptosis in RL95-2 cells was measured by flow cytometry.Western blot analysis was used to test the effect of cinnamaldehyde on the expression of Cleaved caspase-3,caspase-3,NF-κB·p65,IL-6 and IGF-R in RL95-2 cells.【Results】Cinnamaldehyde can reduce the viability rate of endometrial cancer RL95-2 cells,which is related to the treatment duration and concentration. Compared with the solvent control group,the apoptosis percentage of RL95-2 cells in the cinnamaldehyde group(0.29,0.59,1.20 mg/mL) was significantly increased after 48 hours(P < 0.01),typical apoptotic bodies were found,and the expression of Cleaved caspase-3 protein was significantly increased(P < 0.01),there was no significant change in the expression of Caspase 3 protein(P > 0.05),while the expression of NF-κB·p65,IL-6 and IGF-R protein were significantly increased(P <0.05).【Conclusion】Cinnamaldehyde can reduce the expression of NF-κB·p65,IL-6 and IGF-R proteins in RL95-2 cells and promote the apoptosis of RL95-2 cells,thus playing an anti-endometrial cancer role.更多还原