【摘要】 为了明确从罹病烟粉虱Bemisia tabaci上分离的虫生真菌(菌株FZ-01)的分类归属及其生物防治的潜力,采用形态学特征观察和r DNA-ITS序列分析方法对该菌株进行鉴定,并对其生物防治潜力进行评估。结果发现:菌株FZ-01为玫烟色棒束孢Isariafumosorosea;该菌株在马铃薯PDA培养基上生长良好;适应温度和酸碱度范围较广,可耐高温(35℃)和酸碱(pH 4～10);孢子萌发速率快(5 h)且萌发率高(>90%);菌株致病力测定表明分生孢子浓度为1.0×107 cfu/mL时,对烟粉虱(MEAM1)成虫致死中时间(LT50)仅为2.75 d,接菌4 d时致死中浓度(LC50)仅为2.69×104 cfu/mL。综上结果表明,玫烟色棒束孢FZ-01对烟粉虱具有十分优良的生防潜力。更多还原

【Abstract】 A strain of entomopathogenic fungi(FZ-01) isolated from Bemisia tabaci was identified morphologically and using molecular method employing rDNA-ITS gene sequences. The biocontrol potential of the strain was also evaluated. The strain FZ-01 was identified as Isaria fumosorosea, growing well on the potato medium(PDA), showing wide adaptability to temperatures(above 35 ℃) and pH values(pH 4—10), and with fast spore germination speed(5 h) and high spore germination rate(>90%). Under laboratory conditions, the virulence test showed that the strain at conidium concentration of 1.0×107 cfu/mL was characterized by the medium lethal time(LT50) of 2.75 d and the medium lethal concentration(LC50) of 2.69×104 cfu/mL at 4 d after inoculation. The results indicate that the strain FZ-01 possesses promising biocontrol potential against B. tabaci.更多还原