【摘要】 为了研究不同激活状态的巨噬细胞对流行性乙型脑炎病毒（Japanese encephalitis virus,JEV）感染的影响,并明确NO的产生在此过程中的作用,利用细胞因子将小鼠巨噬细胞系RAW264.7诱导成不同激活状态的巨噬细细胞,通过TCID₅₀、免疫荧光技术和实时荧光定量PCR方法比较JEV强毒株NJ2008在不同激活状态的巨噬细胞上的感染情况,并利用实时荧光定量PCR方法分析了调控NO产生的关键分子（iNOS和Agr1）的变化。结果显示,相对于PBS处理组,M1型巨噬细胞显著地抑制JEV的感染并伴随明显增加的iNOS/Arg1比值;而M2型巨噬细胞虽伴随有显著降低的iNOS/Arg1比值,但JEV在M2型巨噬细胞上的感染与PBS组差异无显著统计学意义。结果表明NO的上调表达在M1型巨噬细胞抑制JEV感染中起着主导作用。本研究为进一步揭示巨噬细胞在JEV感染中作用奠定基础。更多还原

【Abstract】 The effect of macrophages with different activated phenotypes on Japanese encephalitis virus（JEV） infection was studied with titration(TCID₅₀), immunofluorescence assay and real-time qPCR. Mouse macrophages were polarized by different cytokines including IFN-γ or IL-4 and the role of NO production during JEV infection was analyzed in terms of expression of iNOS and Arg1 by using realtime qPCR. The results showed that M1 macrophages with increased ratio of iNOS/Arg1 clearly inhibited JEV infection as compared with PBS-treated macrophages. In contrast, M2 macrophages with decreased ratio of iNOS/Arg1 showed no effect on JEV infection in comparison of PBS-treated controls. These results demonstrated that macrophages with different polarized phenotypes played different roles in JEV infection. Furthermore, the up-regulation of NO in M1 macrophages mainly contributed to inhibition of JEV infection. Thus,this study provided the basis of better understanding the role of macrophages in JEV infection.更多还原