【摘要】 目的:探讨RCAS1/EBAG9与P53蛋白在肝内胆管癌(ICC)组织中的表达及临床意义。方法:用免疫组化法检测41例ICC组织及9例正常胆管组织中RCAS1/EBAG9和P53蛋白的表达,分析RCAS1/EBAG9与P53蛋白的表达与ICC患者的临床病理因素和预后的关系。结果:RCAS1/EBAG9与P53蛋白在ICC组织中阳性率均明显高于与正常胆管组织(均P<0.05);RCAS1/EBAG9蛋白表达与肿瘤大小、病理分级、TNM分期以及淋巴结转移有关(均P<0.05),而P53蛋白表达与肿瘤大小、病理分级以及TNM分期有关(均P<0.05)。ICC中RCAS1/EBAG9与P53蛋白表达正相关(r=0.329,P=0.018)。RCAS1/EBAG9与P53蛋白阴性表达患者术后总生存期明显长于RCAS1/EBAG9与P53蛋白阳性表达患者(χ~2=3.862,P=0.049;χ~2=4.977,P=0.026)。单因素与多因素Cox回归分析表明,RCAS1/EBAG9蛋白表达是ICC患者预后的独立危险因素(HR=3.657,95%CI=1.111～12.040,P=0.033)。结论:RCAS1/EBAG9与P53蛋白在ICC组织中表达增加,并且与ICC患者不良临床病理特征及预后密切相关,其中RCAS1/EBAG9可能起了关键作用。更多还原

【Abstract】 Objective: To investigate the protein expressions of RCAS1/EBAG9 and P53 in intrahepatic cholangiocarcinoma(ICC) tissue and their clinical significance.Methods: The protein expressions of RCAS1/EBAG9 and P53 in 41 specimens of ICC tissue and 9 specimens of normal bile duct tissue were detected by immunohistochemcal staining. The relations of RCAS1/EBAG9 and P53 protein expressions with the clinicopathologic characteristics and prognosis of the patients were analyzed.Results: The positive expression rates of both RCAS1/EBAG9 and P53 proteins in ICC tissue were significantly higher than those in normal bile duct tissue(both P<0.05). The RCAS1/EBAG9 protein expression was significantly related to tumor size, pathological grade, TNM stage and lymph node metastasis, while P53 protein expression was significantly associated with tumor size, pathological grade, and TNM stage(all P<0.05). There was a positive correlation between RCAS1/EBAG9 and P53 protein expression in ICC tissue(r=0.329, P=0.018).The overall postoperative survival time in patients with negative RCAS1/EBAG9 or P53 protein expression was significantly longer than that in patients with positive RCAS1/EBAG9 or P53 protein expression(χ~2=3.862,P=0.049; χ~2=4.977, P=0.026). Results of univariate and multivariate Cox regression analysis showed that RCAS1/EBAG9 protein expression was an independent risk factor for the prognosis of ICC patients(HR=3.657, 95% CI=1.111–12.040, P=0.033).Conclusion: The RCAS1/EBAG9 and P53 protein expressions are increased in ICC tissue, and are closely associated with unfavorable clinicopathologic features and outcomes of the ICC patients, in which, the RCAS1/EBAG9 may probably play a causal role.更多还原