【摘要】 目的:探讨脂质体华卟啉钠（DVDMS-L）的制备及其对胆管癌细胞的光动力杀伤效应。方法:采用薄膜-水化法制备DVDMS-L,并检测其粒径、电位及包封率。分别用相同浓度梯度（0、0.06、0.12、0.25、0.5、1μmol/L）的DVDMS-L与游离华卟啉钠（DVDMS）,光照强度10J/cm²条件下,作用于胆管癌HICC-9810细胞4h后,用CCK8法检测两者对人胆管癌HICC-9810细胞的光动力杀伤效果。结果:所制得的DVDMS-L平均粒径为（113.7±14.2）nm,Zeta电位为-（24.5±3.2）mV,包封率为（61.74±1.49）%;在光照条件下,HICC-9810细胞在DVDMS-L与DVDMS处理后存活率均明显降低,并浓度依赖性,但相同浓度（0.12～1μmol/L）下的DVDMS-L的作用明显强于DVDMS,两者的IC₅0分别为0.11、0.29μmol/L（均P<0.05）。结论:成功制备DVDMS-L,其对人胆管癌细胞的体外光动力杀伤效应优于游离DVDMS。更多还原

【Abstract】 Objective: To investigate the preparation of liposomal sinoporphyrin sodium（DVDMS-L） and its photodynamic killing effect against cholangiocarcinoma cells. Methods: The DVDMS-L particles were prepared by thin-film hydration technique, and the particle size and zeta potential, and entrapment efficiency were determined. The cholangiocarcinoma HICC-9810 cells were exposed to the same concentration gradient（0, 0.06, 0.12, 0.25, 0.5 and 1 μmol/L） of DVDMS-L or free sinoporphyrin sodium（DVDMS） under a light dose irradiation of 10 J/cm² for 4 h, and then, the photodynamic killing effects of DVDMS-L and DVDMS on HICC-9810 cells were determined by CCK8 assay.Results: Of the prepared DVDMS-L, the average particle size was（113.7±14.2） nm, Zeta potential was –（24.5±3.2） mV, and encapsulation efficiency was（61.74±1.49） %. The viability of HICC-9810 cells was significantly decreased after exposure to either DVDMS-L or DVDMS in a concentration-dependent manner, where the effect of DVDMS-L was significantly greater than that of DVDMS under the same concentration（0.12– 1 μmol/L）, with the IC₅0 of 0.19 and 0.42 μmol/L respectively（all P<0.05）.Conclusion: DVDMS-L is successfully prepared and its photodynamic effect against cholangiocarcinoma cells is superior to that of DVDMS.更多还原