【摘要】 为了探讨外源亚精胺对低温条件下菜豆种子萌发与种子耐低温性的影响。本研究以菜豆品种‘热那亚’为试验材料,设置常温对照（CK）、4℃低温（LT）以及4℃低温条件下添加1 mmol/L Spd （LT+Spd） 3个处理组,对其发芽率、发芽指数、种子活力及生理指标进行测定。结果显示:4℃低温条件下,添加1 mmol/L的Spd后菜豆种子发芽率、发芽指数和种子活力分别提高了95.69%、136.10%和217.12%;电解质外渗率降低;抗氧化酶SOD、POD、CAT的活性升高;H₂O₂、O₂^-、MDA、脯氨酸和可溶性蛋白的含量降低。由此可见,施用外源亚精胺能够缓解低温胁迫对菜豆种子造成的伤害,提高菜豆种子的耐低温性,从而提高菜豆种子的发芽率、发芽指数和种子活力。更多还原

【Abstract】 In order to study the effect of exogenous Spd on seed germination and low temperature tolerance of snap bean under low temperature, the snap bean cultivar‘Renaya’was used as experimental material. Three treatments, normothermia control（CK）, 4℃ low temperature treatment（LT） and addition of 1 mmol/L Spd at 4℃（LT+Spd） were used to determine the germination rate, germination index, seed vigor and physiological index.The results showed that the germination rate, germination index and seed vigor increased by 95.69%, 136.10%and 217.12%, respectively, after adding 1 mmol/L Spd at 4℃; the electrolyte leakage rate decreased, the activities of antioxidant enzymes SOD, POD and CAT increased, and the contents of H₂O₂, O₂^-, MDA, proline and soluble protein decreased. Therefore, the application of exogenous Spd can alleviate the damage caused by low temperature stress and improve the low temperature tolerance of snap bean seeds,, thus to enhance the germination rate, germination index and seed vigor of snap bean seeds.更多还原