【摘要】 明确抗性生物型野燕麦对高效氟吡甲禾灵产生抗性的3种酶机制,以期为治理与延缓杂草抗药性提供科学依据。采用温室盆栽法,分别测定了抗性和敏感性生物型野燕麦乙酰辅酶A羧化酶(ACCase)、细胞色素P450(CYP450)还原酶和谷胱甘肽-S-转移酶(GSTs)的活性差异。结果表明,未加药剂时,抗性生物型野燕麦ACCase的活性低于敏感性生物型;随着药剂浓度的增加,2个生物型ACCase的活性逐渐降低,但抗性生物型降低幅度小于敏感生物型,二者ACCase活性IC50分别为78.369μmol/L和43.469μmol/L,活性倍数1.8。清水处理时,野燕麦抗性生物型与敏感生物型CYP450还原酶和GSTs活性差异均不明显;施药后,抗性生物型CYP450还原酶和GSTs的活性均高于敏感生物型,药后12天,抗性生物型与敏感生物型CYP450还原酶活性分别113.1 pmol/L和38.9 pmol/L,活性倍数2.91;2个生物型GSTs活性分别为210.6 mIU/L和319.1 mIU/L,活性倍数1.52。靶标酶活性较高水平的维持及代谢酶活性的增强是野燕麦对高效氟吡甲禾灵产生抗性的重要机制。更多还原

【Abstract】 We verified three enzyme mechanisms of resistant biotype Avena fatua L.against Haloxyfop-Pmethyl,in order to provide a basis for controlling and delaying weed resistance.We tested the difference of ACCase,CYP450 reductase and GSTs activities in Haloxyfop-R-methyl resistant and sensitive Avena fatua L.biotypes by potting method in greenhouse.The results showed that:the ACCase activity of resistant biotype Avena fatua L.was lower than that of sensitive biotype when herbicides were not applied;with the increase of Haloxyfop-R-methyl concentration,the activity of 2 biotype ACCase decreased gradually,but the decrease of theresistantbiotypewaslessthanthatofthesensitivebiotype,thetwoACCaseactivityIC50 valuewas78.369μmol/L and 43.469 μmol/L,respectively,and the active multiple was 1.8;there was no significant differences in the activity of CYP450 reductase and GSTs between the resistant biotype and sensitive biotype of Avena fatua L.under water treatment;the activity of the resistant bio-type CYP450 reductase and GSTs was higher than that of the sensitive biotype after the application;after 12 days of application,the content of the resistant biotype and the sensitive biotype CYP450 reductase was 113.1 pmol/L and 38.9 pmol/L,respectively,the activity multiple was 2.91,and the 2 biotypes’ GSTs activity was 210.6 mIU/L and 319.1 mIU/L,respectively,and the active multiple was 1.52.The high level of target enzyme activity and the enhancement of metabolic enzyme activity are important mechanisms for the resistance of Avena fatua L.to Haloxyfop-P-methyl.更多还原