【摘要】 本研究对实验室购买、收集的9株红曲菌株分泌麦角固醇、色素和桔霉素的能力进行比较分析,并探讨了三者的相关性,旨在为红曲菌资源的深度挖掘提供基础信息。分别采用UPLC-紫外检测法、UV-VIS法和UPLC-荧光检测法测定9株红曲菌发酵制备红曲样品中麦角固醇、色价和桔霉素的含量;在此基础上,采用统计学分析方法分析了三者之间的相关性。分析表明,不同菌株产麦角固醇、色素和桔霉素的能力不同,其中紫色红曲菌OUT 2011发酵红曲中麦角固醇含量和色价均最高,分别达到(1121.96±55.06) mg/kg和(1058.97±5.04) U,安卡红曲菌AS 3.554发酵红曲中麦角固醇含量和色价均最低,而从毛红曲菌OUT 2137、红色红曲菌AS 3.549,安卡红曲菌AS 3.554、发白红曲菌CICC5017、橙色红曲菌CICC5015发酵的红曲均未检出桔霉素。9株红曲菌发酵产麦角固醇、色素和桔霉素能力各不相同;相关性分析显示菌株分泌的麦角固醇和色素水平呈正相关性,为筛选高产麦角固醇的红曲菌株提供了参考。更多还原

【Abstract】 9 Monascus strains stored in the lab were compared to test their abilities to produce ergosterol,pigment and citrinin for further utilizing Monascus strain in the future. UPLC with UV detector, UV-VIS and UPLC with fluorescence detector were adopted to measure ergosterol, pigment and citrinin produced by these 9 strains, and the correlation of these 3 compounds were evaluated by statistical methods. The results revealed that the 9 Monascus strains had different abilities to produce ergosterol, pigment and citrinin. Among them,OUT 2011(M. purpureus) had the strongest ability to produce ergosterol and pigment, amounting to(1121.96±55.06) mg/kg and(1058.97 ± 5.04) U, respectively; AS 3.554(M. anka) had the lowest ability to produce ergosterol and pigment. No citrinin was detected in red yeast rice made by strains of OUT2137(M. pilosus), AS3.549(M. ruber), AS 3.554(M. anka), CICC5017(M. albidus) and CICC5015(M. aurantiacus). The production of ergosterol was positively correlated with the production of pigment. The putative correlation between ergosterol and pigment laid a foundation for screening strain with high production of ergosterol.更多还原