【摘要】 目的:探讨HOXB5基因调控STAT3信号对膀胱癌细胞凋亡及免疫抑制因子影响及机制研究。方法:以正常膀胱上皮细胞SV-HUC-1为对照,Western blot检测膀胱癌T24、5637和BIU-87细胞HOXB5的蛋白表达。将HOXB5的特异性siRNA(si-HOXB5组)转染T24细胞,同时转染无干扰的siRNA(NC组),并设置空白组,AG490作为STAT3信号通路抑制剂,48 h后,流式细胞术检测各组细胞凋亡率; Western blot检测各组细胞p-JAK2、p-STAT3、STAT3、Bax、VEGF和TGF-β1蛋白表达。结果:HOXB5在T24、5637和BIU-87细胞的蛋白表达均显著高于在SV-HUC-1细胞中的表达(P<0. 05)。转染si-HOXB5的T24细胞HOXB5蛋白表达显著低于空白组(P<0. 05)。与NC组比较,si-HOXB5组细胞凋亡率显著升高,p-JAK2、pSTAT3、VEGF和TGF-β1的蛋白表达显著降低,Bax的蛋白表达显著升高(P<0. 05)。si-HOXB5+AG490组细胞凋亡率显著高于si-HOXB5组和AG490组(P<0. 05)。结论:HOXB5基因表达抑制可诱导膀胱癌细胞凋亡,下调免疫抑制因子VEGF和TGF-β1的表达,其机制与抑制STAT3信号通路有关。更多还原

【Abstract】 Objective: To investigate the effect of HOXB5 gene on apoptosis and immunosuppressive factors in bladder cancer cells through regulation of STAT3 signaling. Methods: Normal bladder epithelial cells SV-HUC-1 were used as controls,and Western blot was used to detect the protein expression of HOXB5 on T24,5637 and BIU-87 cell of bladder cancer. The specific siRNA of HOXB5( si-HOXB5 group) was transfected into T24 cells,and the siRNA without interference( NC group) was transfected,and the blank group was set up. AG490 was used as a STAT3 signal pathway inhibitor. After 48 h,the apoptosis rate of each group was detected by flow cytometry. The protein expression of p-JAK2,p-STAT3,Bax,VEGF and TGF-β1 in each group were detected by Western blot. Results: The protein expression of HOXB5 in T24,5637 and BIU-87 cells was significantly higher than that in SV-HUC-1 cells( P<0. 05). The expression of HOXB5 protein in T24 cells transfected with si-HOXB5 was significantly lower than that in blank group( P<0. 05). Compared with the NC group,the apoptosis rate increased significantly in si-HOXB5 group. The protein expression of pJAK2,p-STAT3,VEGF and TGF-β1 decreased significantly,and the protein expression of Bax increased significantly( P<0. 05). The apoptotic rate in si-HOXB5+AG490 group was significantly higher than si-HOXB5 group and AG490 group( P <0. 05). Conclusion:The inhibition of HOXB5 gene expression can induce the apoptosis of bladder cancer cells,down regulate the expression of VEGF and TGF-β1,and the mechanism is related to the inhibition of STAT3 signaling pathway.更多还原